Mouse monoclonal antibody igg against rat neurofilament 200

To assess effects on neuronal survival, SG explants were cultured as described above for 72 h, except that the explants were grown on glass cover slips. The explants were fixed as above and treated with 0.5% peroxide in methanol to block endogenous peroxidases. Then explants were treated with a mouse monoclonal antibody IgG against rat neurofilament 200 (Sigma- Aldrich) followed by a biotinylated secondary anti-mouse IgG and developed by an avidin and DAB procedure (Vector Laboratories, Burlingame, CA, USA). The tissue was cleared with citrosol (Fisher Scientific, Waltham, MA, USA) to allow the visualization of the cell somas for evaluation of neuronal survival. 8 SG explants were studied for each condition.

To assess effects on neuronal survival, half turn SG explants were cultured as above for 72 hours, except that the explants were grown on glass cover slips. The explants were fixed as above, treated with 0.5% peroxide in methanol to block endogenous peroxidases, reacted with a mouse monoclonal antibody IgG against rat neurofilament 200 (Sigma-Aldrich), followed by a biotinylated secondary anti-mouse IgG, and developed by an avidin and DAB procedure (Vector Laboratories, Burlingame, CA). The tissue was cleared with citrosol (Fischer Scientific, Waltham, MA, USA) to allow visualization of the cell somas for evaluation of neuronal survival. 12 SG explants were studied per condition.