TOPK (sc-293028) and p-AKT (sc-7985) were purchased from Santa Cruz Technology, Inc. (Santa Cruz, CA). β-Actin (ET1701-80) was purchased from Hangzhou HuaAn Biotechnology, Inc. (Hangzhou, ZJ). Antibodies to detect COX2 (#4842), MET (#8198), p-MET (#3077), p-ERK (#9101), ERK (#9102), AKT (#9272), p-c-Jun (#2361), c-Jun (#9165), PARP (#9542), c-PARP (#5625), His (#2365), HA (#3724), and p-Tyrosine (#8954) were purchased from Cell Signaling Technology (Danvers, MA). Horseradish peroxidase-labeled goat anti-mouse IgG (H + L) (E031110) and goat anti-rabbit IgG (H + L) (E031120) were purchased from EarthOx, LLC (San Francisco, CA). Phospho-TOPK at Y74 antibody was prepared by Abgent, Inc. (Suzhou, JS). All antibodies were used following the instructions of the respective manufacturers.
Gentamicin,l -glutamine, epidermal growth factor, HGF, Tris, NaCl, and SDS for molecular biology were purchased from Sigma-Aldrich (St. Louis, MO). U46619, Seratrodast, and Ozagrel HCl were all obtained from ApexBio Technology, LLC (Houston, TX). Celecoxib was purchased from Pfizer (New York, NY). Gefitinib was purchased from Gold Biotechnology (St. Louis, MO). Pantoprazole was obtained from Kaifu Co., Ltd (Wuxi, JS). SR11302 and PGE2 were purchased from Selleckchem, Inc. (Houston, TX).
Gentamicin,