Cultured T cells were washed in PBS/0.1% BSA and re-suspended at 1×106 cells in 50 uL Brilliant Buffer (BD Biosciences) supplemented with 4% rat serum for 15 minutes at 4C. Cells were then incubated for 30 minutes at 4°C in 100 μL of Brilliant Buffer using the following antibody fluorophore conjugates (all from BD Biosciences unless otherwise noted): CD7 BV421, CD4 BV510, CCR4 BV605 (BioLegend), CD8 BV650, CD196 BV786 (BioLegend), CD3 AF488, CD45RA PerCPCy5.5, CD183 PE, CD197 PE-CF594, CD185 PE-Cy7 (BioLegend), and CCR10 APC (R&D Systems). Full details of fluorophore conjugated antibodies can be found in supplemental table 5 . Cells were then washed twice in PBS/0.1% BSA and data acquired on a ZE5 (Yeti) cytometer (BioRad/Propel Labs). Compensation and analyses were performed on FlowJo V10 (TreeStar) using fluorescence minus one (FMO) controls. Statistical analyses were performed on GraphPad Prism 7 using 2-way ANOVA with Bonferroni post-hoc corrections.
Cd196 bv786
Manufactured by BioLegend
The CD196 BV786 is a fluorescence-labeled antibody used for flow cytometry applications. It recognizes the human chemokine receptor CCR6, which is expressed on various immune cell types. This product can be used to identify and characterize CCR6-positive cells in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Cd8 bv650, by BioLegend (2 mentions)
Ccr10 apc, by R&D Systems (2 mentions)
Cd4 bv510, by BioLegend (2 mentions)
Ccr4 bv605, by BioLegend (2 mentions)
Cd3 af488, by BioLegend (2 mentions)
Cd45ra percp cy5, by BioLegend (2 mentions)
Cd183 pe, by BioLegend (2 mentions)
Brilliant buffer, by BD (2 mentions)
Cd7 bv421, by BioLegend (2 mentions)
Cd197 pe cf594, by BioLegend (2 mentions)
Cd185 pe cy7, by BioLegend (2 mentions)
2 protocols using cd196 bv786
1
Multiparameter Flow Cytometry of T Cells
2
Multiparameter Flow Cytometry of T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cultured T cells were washed in PBS/0.1% BSA and re-suspended at 1×106 cells in 50 uL Brilliant Buffer (BD Biosciences) supplemented with 4% rat serum for 15 minutes at 4C. Cells were then incubated for 30 minutes at 4°C in 100 μL of Brilliant Buffer using the following antibody fluorophore conjugates (all from BD Biosciences unless otherwise noted): CD7 BV421, CD4 BV510, CCR4 BV605 (BioLegend), CD8 BV650, CD196 BV786 (BioLegend), CD3 AF488, CD45RA PerCPCy5.5, CD183 PE, CD197 PE-CF594, CD185 PE-Cy7 (BioLegend), and CCR10 APC (R&D Systems). Full details of fluorophore conjugated antibodies can be found in supplemental table 5 . Cells were then washed twice in PBS/0.1% BSA and data acquired on a ZE5 (Yeti) cytometer (BioRad/Propel Labs). Compensation and analyses were performed on FlowJo V10 (TreeStar) using fluorescence minus one (FMO) controls. Statistical analyses were performed on GraphPad Prism 7 using 2-way ANOVA with Bonferroni post-hoc corrections.
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