Anti mettl3

Manufactured by Merck Group

Anti-METTL3 is a laboratory product developed by Merck Group. It functions as an inhibitor of the METTL3 enzyme, which is involved in the process of N6-methyladenosine (m6A) modification of RNA. The core function of Anti-METTL3 is to enable the study and analysis of the role of m6A modification in various biological processes.

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2 protocols using anti mettl3

Western Blotting and Co-IP Protocol

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Plasmids are described in Supplementary Table S1. The following antibodies and reagents were used for western blotting assay, immunofluorescence, or Co-IP: rabbit anti-GAPDH antibody (Catalog#/Clone: SAB2701826/polyclonal), rabbit anti-GFP antibody (Catalog#/Clone: AB10145/polyclonal), anti-METTL14 antibody (Catalog#/Clone: SAB5700855/ polyclonal), anti-METTL3 (Catalog#/Clone: AV34590) were purchased from Sigma-Aldrich; anti-EsxB antibody (Catalog#/Clone: ab45074) and Anti-ESAT6 antibody (Catalog#/Clone: ab45073) were from Abcam; anti-CYBB-NOX2 antibody (Catalog#/Clone: NBP2-38642/polyclonal) was from Novusbio; rabbit anti-phospho-p38 antibody (Catalog#/Clone: 9215/3D7), anti-p38 MAPK (Catalog#/Clone: 8690/D13E1), were from Cell Signaling Technology, Danvers, MA; anti-YTHDF2 antibody (Catalog#/Clone: 24744-1-AP/polyclonal) was purchased from Proteintech; mouse monoclonal antibody to Mettl14 phosphorylated at Thr72 (p-T72-METTL14) was generated by immunization of rabbits, in collaboration with AbClonal Biotech.

Ma M., Duan Y., Peng C., Wu Y., Zhang X., Chang B., Wang F., Yang H., Zheng R., Cheng H., Cheng Y., He Y., Huang J., Lei J., Ma H., Li L., Wang J., Huang X., Tang F., Liu J., Li J., Ying R., Wang P., Sha W., Gao Y., Wang L, & Ge B. (2024). Mycobacterium tuberculosis inhibits METTL14-mediated m6A methylation of Nox2 mRNA and suppresses anti-TB immunity. Cell Discovery, 10, 36.

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Generation and Characterization of ALKBH5 Mutants

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The pCDH-Strep-ALKBH5 expression plasmid was generated by cloning the corresponding coding sequence into pCDH-Strep vector. All the pCDH-Strep-ALKBH5 K/R (lysine to arginine) or S/A (serine to alanine) mutants were derived from pCDH-Strep-ALKBH5 by site-directed mutagenesis. All expression plasmids for the SUMO systems were kindly provided by Dr. Jiemin Wong's lab. Antibodies used in this study were listed as follows: anti-m⁶A (Synaptic Systems# 202003), anti-γ H2A.X (CST#9719S), anti-γ H2A.X (Thermo Fisher#MA1-2022), anti-ALKBH5 (Sigma#HPA007196), anti-ALKBH5 (Thermo Fisher #703570), anti-FTO (Sigma#SAB2106776), anti-METTL3 (Sigma#SAB2104747), anti-METTL14 (Sigma#HPA038002), anti-SUMO-1 (Thermo Fisher #33–2400), anti-SUMO-2/3 (CST#4971P), anti-ERK (CST#9102S), anti-p-ERK (CST#9106S), anti-JNK (CST#9252S), anti-p-JNK (CST#4671S), anti-phophoserine (Sigma#P5747), anti-phophotyrosine (Sigma#SAB5200015), anti-Strep (Sigma#SAB2702216), anti-Annexin V (Thermo Fisher #17800774), anti-Actin (CST#8457S), anti-Tubulin (CST#2146S), anti-Lamin A/C (CST#4777S), anti-HA (CST#2362), anti-Flag (Sigma#F1804), anti-IGF2BP2 (CST#14672S), anti-eIF3A (CST#3411S).

Yu F., Wei J., Cui X., Yu C., Ni W., Bungert J., Wu L., He C, & Qian Z. (2021). Post-translational modification of RNA m6A demethylase ALKBH5 regulates ROS-induced DNA damage response. Nucleic Acids Research, 49(10), 5779-5797.

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