Rabbit anti iκbα antibody

Rabbit anti-phospho-IκBα antibody, rabbit anti-IκBα antibody, and rabbit anti-β-actin antibody were obtained from Cell Signaling Technology (Beverly, MA). Rabbit anti-NF-κB p65 antibody, anti-Bcl-2 antibody, and anti-Histon H1 antibody were purchased from Santa Cruz Biotechnology (Lake Placid, NY). Rabbit anti-cyclin D1 antibody, anti-cyclin D2 antibody, anti-cyclin D3 antibody, and anti-cyclin E antibody were purchased from PharMingen (San Diego, CA). Horseradish peroxidase-conjugated secondary antibodies were obtained from Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA). IMD-0354, an IKKβ inhibitor 20 (link)–22 , was kindly provided by the Institute of Medicinal Molecular Design Inc. (Tokyo, Japan). Pemetrexed and cisplatin were purchased from LKT Laboratories, Inc. (St. Paul, MN).

Cells were added into cytoplasmic and nuclear extracts by NE-PER ® Nuclear and Cytoplasmic Extraction Reagents. Protein concentrations of the extracts were measured by BCA assay. Rabbit anti-IκBα antibody was purchased from Cell Signaling Technology. Rabbit anti-β-Actin antibody and anti-Histone 3 antibody were purchased from Abcam (Catalog number ab8227, ab1791). Mouse anti-NF-κB p65 subunit antibody was purchased from Santa Cruz. Western blot analyses were performed as previously described (55 (link)). Actin level in cytoplasmic extraction and Histone 3 in nuclear extraction were detected to show equal protein loading.