Total RNA was isolated from the middle lobe of mouse lung and cultured cells using TRlzol reagent (Takara, Dalian, China). RNA quantity and quality were measured using a NanoDrop 2000 spectrophotometer (Thermo Scientific, MA, USA). Complementary DNA synthesis was performed using a Moloney murine leukaemia virus reverse transcriptase kit (Invitrogen, CA, USA). Quantitative RT-PCR was performed using the SYBR Premix Ex Taq (Takara), and the relative expression of each target gene was normalised by Actb expression, as described previously [25 (link)]. The primers used for each target gene are provided in supplementary table S2 .
Moloney murine leukaemia virus reverse transcriptase kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Moloney murine leukaemia virus reverse transcriptase kit is a lab equipment product that provides reverse transcriptase enzyme for the conversion of RNA to complementary DNA (cDNA). The kit contains the necessary components to perform this enzymatic reaction.
Automatically generated - may contain errors
Lab products found in correlation
Nanodrop 2000, by Thermo Fisher Scientific (1 mentions)
Sybr premix ex taq, by Takara Bio (1 mentions)
Trlzol reagent, by Takara Bio (1 mentions)
Steponeplus real time pcr system, by Thermo Fisher Scientific (1 mentions)
Faststart sybr green master mix, by Thermo Fisher Scientific (1 mentions)
Lightcycler system, by Roche (1 mentions)
2 protocols using moloney murine leukaemia virus reverse transcriptase kit
1
Quantifying Gene Expression in Mouse Lung
2
Quantitative RT-PCR Gene Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
First-strand cDNA was synthesized from DNase I-treated total RNA templates that were primed with oligo-dT using the Moloney-murine-leukaemia virus-reverse transcriptase kit from Invitrogen (San Diego, CA). Quantitative RT-PCR was performed using the Light Cycler system (Roche Diagnostics, Mannheim, Germany) or StepOnePlusTM Real-Time PCR System with FastStart SYBR green master mix (Applied Biosystems, Foster City, CA). The primer sequences for PCR amplification are shown in Table 2 . The PCR products were quantified by fit-point analysis, and results were normalized to β-actin or cyclophilin.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!