Western blot assays were performed as previously described (Jin et al., 2018 (link)). Briefly, total cellular or sEV proteins were obtained using RIPA lysis buffer (P0013B, Beyotime) and quantified with a BCA Protein Assay Kit (P0012, Beyotime). The proteins were electrophoresed by 6%–12% sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and subsequently incubated with primary antibodies against TSG101 (1:1000, Abcam), HSP70 (1:1000, Abcam), CD9 (1:1000, Abcam), CD63 (1:500, Bimake, China), calnexin (1:1000, Abcam), SLC6A8 (1:1000, SAB, USA), ATAG (1:1000, Abcam), GAMT (1:1000, Abcam), CKB (1:1000, Abcam), NPM1mA (1:1000, Thermo Fisher Scientific), PABPC1 (1:1000, SAB), KHSRP (1:1000, Abcam), β‐actin (1:1000, ZSGBBIO, China). β‐actin was detected as a loading control. The appropriate HRP‐conjugated anti‐mouse or anti‐rabbit IgG (ZSGBBIO) was used as a secondary antibody. Protein signals were visualized using an enhanced chemiluminescence system (Amersham Pharmacia Biotech, Japan).
Hrp conjugated anti mouse or anti rabbit igg
Manufactured by ZSGB-BIO
Sourced in United States
HRP-conjugated anti-mouse or anti-rabbit IgG is a secondary antibody that is conjugated with horseradish peroxidase (HRP). It is designed for use in immunoassays and other applications that require the detection of primary antibodies targeting mouse or rabbit immunoglobulin G (IgG).
Automatically generated - may contain errors
Lab products found in correlation
Enhanced chemiluminescence system, by Cytiva (1 mentions)
Khsrp, by Abcam (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
Hsp70, by Abcam (1 mentions)
Tsg101, by Abcam (1 mentions)
Calnexin, by Abcam (1 mentions)
β actin, by ZSGB-BIO (1 mentions)
Basic fibroblast growth factor (bfgf), by Proteintech (1 mentions)
Anti cd44, by Proteintech (1 mentions)
Anti klf4, by Proteintech (1 mentions)
Ultra low attachment plate, by Corning (1 mentions)
Fitc labeled goat anti rabbit igg, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Anti pcna, by Bioworld Technology (1 mentions)
Anti gapdh, by Bioworld Technology (1 mentions)
C myc, by Abcam (1 mentions)
β catenin, by Abcam (1 mentions)
Anti β actin, by ZSGB-BIO (1 mentions)
2 protocols using hrp conjugated anti mouse or anti rabbit igg
1
Western Blot Analysis of Extracellular Vesicle Proteins
2
Antibody-Based Protein Detection Protocol
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The antibodies specifically against SOX2, c-MYC, 14-3-3, and β-Catenin were purchased from Abcam (UK); anti-CD44 and anti-KLF4 were products of Proteintech (USA); anti-β-actin, HRP-conjugated anti-mouse or anti-rabbit IgG were purchased from ZSGBBIO (CHN); anti-CBY1 was purchased from Santa Cruz (USA); anti-SP3 was purchased from Sigma-Aldrich (USA); anti-Nanog, anti-phosphorylated-β-Catenin, anti-GAPDH and anti-PCNA were obtained from Bioworld (USA); and FITC-labeled goat anti-rabbit IgG was from Sigma-Aldrich (USA). BSA was a product of Sigma-Aldrich (USA); bFGF was obtained from Proteintech (USA); EGF and B27 supplement were from Invitrogen (Thermo Fisher, USA), and ultra-low attachment plates were purchased from Corning Corp. (USA).
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