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10 protocols using tetric n flow

1

MgO Nanoparticles Enhance Composite Restoration

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The following two composite materials were evaluated: Tetric N-Flow and Tetric N-Ceram (Ivoclar Vivadent). Magnesium oxide nanoparticles wires and spheres were synthesized and coated with zein polymer as described earlier (Table 1) [34 ].

Manufacturer and composition of materials used in this study

Material (Manufacturer)Composition
Tetric N-Ceram, Ivoclar Vivadent AG, Schaan, LiechtensteiDimethacrylates (19–20 wt. %), Fillers contain barium glass, ytterbium trifluoride, mixed oxide and copolymers (80–81 wt. %)
Tetric N-Flow, Ivoclar Vivadent AG, Schaan, LiechtensteinUDMA, bis-GMA, TEGDMA
zMgO Nanoparticles (Lab prepared)MgO Nanoparticles (Nanowires and Nanospheres) coated with zein nanopolymer
In this study, a conventional flowable composite (Tetric-N flow, Ivoclar/Vivadent, Liechtenstein), two-step self-etch adhesive (Clearfil SE bond 2, Kuraray Noritake Dental, Japan) and MgO nanowires (particle size 40nm diameter and 100nm length) synthesized by microwave in the two different concentrations were used [30 (link)]. The MgO nanofillers were weighed using a balance accurate to 0.0001g (BEL Engineering, Monza, Italy) and were added to the flowable composite in the ratio of 0.3% and 0.5% by weight.
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2

Cranial Window for Two-Photon Imaging

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For two-photon imaging and single-cell electroporation, a cranial window was made over vermis VI of the cerebellum. Anesthesia was induced with 2% isoflurane (Pfizer Japan, Tokyo) in air and maintained at a level of 0.7% during surgery, electroporation, and imaging. The body temperature was continuously monitored and maintained by a heated pad at 38 °C. Both eyes were covered with ophthalmic ointment. Dexamethasone sodium phosphate (4.8 mg/kg) was subcutaneously injected 1–2 hrs before craniotomy to prevent inflammation. To fix an animal head during the electroporation and the two-photon imaging, a head plate made of carbon fiber was implanted on the skull using Optibond (Kerr) and Tetric N-Flow (Ivoclar Vivadent) after injecting a local anesthetic (1% xylocaine) and an analgesic (flunixin meglumine, 2.5 mg/kg). The plastic chamber for applying artificial cerebrospinal fluid (aCSF) was attached on it. Then a rectangle cranial window (3 mm × 2 mm) was made over the cerebellar vermis VI using a dental drill. 2% agarose dissolved in saline was applied to the cranial window before targeted single-cell electroporation to minimize brain movement.
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3

Evaluation of Dental Restorative Materials

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This study was carried out after taking Ethical and Scientific Committee approval (DPU/1184/41/2019) by Dr D Y Patil Vidyapeeth (DPU) (Deemed to be University), Pune, and Environmental Science Technology Study Centre of Bapuji Institute of Engineering and Technology, Davangere, Karnataka, India. The materials used in this study were R1: Jen Rainbow composite (Jen Dent Ukraine), R2: Tetric® N-Flow (Ivoclar Vivadent), and R3: RMGIC Fuji II LC (GC Corporation) (Figs 1 to 3).
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4

Comparative Evaluation of Dental Sealants

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Sixty intact maxillary/mandibular premolar teeth extracted for orthodontic reasons without any carious lesion were included in the study. After collection of teeth samples, saliva and blood were cleaned and brushed. Extraneous soft tissue, superficial debris, and calculus were removed from the teeth with an ultrasonic scaler. Pretreatment of sample teeth was done by giving a prophylaxis with an aqueous pumice slurry, using a prophy cup. Later, samples were washed thoroughly with water and dried. The samples were kept in normal saline at ambient temperature to prevent them from becoming brittle because of dehydration.
The samples were arbitrarily categorized into four groups of 15 teeth:
Group I: Fifteen teeth specimens to be sealed with classical pit fissure sealant (Helioseal F; Ivoclar Vivadent AG, Schaan, Liechtenstein).
Group II: Fifteen teeth specimens for application of flowable composite (Te-Econom Flow; Ivoclar Vivadent AG).
Group III: Fifteen teeth specimens for application of flowable nanocomposite (Tetric N-Flow; Ivoclar Vivadent AG).
Group IV: Fifteen teeth specimens to be sealed with glass ionomer-based sealant (Fuji VII; GC Corporation, Tokyo, Japan).
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5

Flowable Composite Resin Reinforcement

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The primer and/or bond resin was applied to the etching surface following the manufacturer's instruction (Figure 4(a)). The flowable composite resin (Tetric® N-Flow, Ivoclar Vivadent AG, Schaan, Liechtenstein) or composite resin cement (ParaCore®, COLTENE, Coltène/Whaledent AG, Altstätten, Switzerland) was then injected around the contact area (Figures 4(b) and 4(c)), enforcing the tension band with the cerclage wire as described above for about 30 s to squeeze the paste of composite resin into the contact area (Figure 4(d)). The tensile stress was then relieved, and redundancy was removed immediately with a point probe (Figure 4(e)). The restoration was polymerised by light curing from all aspects: occlusal, buccal, and lingual surfaces (Figure 4(f)).
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6

Standardized Class II MOD Cavity Preparation

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Prior to cavity preparation, a flowable composite (Tetric N-Flow, Ivoclar Vivadent, Liechtenstein) was employed to take an imprint for the occlusal surface of each tooth to minimize the requirement for finishing and polishing by restoring each tooth back to its original occlusal anatomy [28 ].
Each tooth then received a standardized class II MOD cavity preparation (3 mm buccolingual width and 6 mm occlusal depth) assessed from the tip of the buccal cusp without a proximal step. For cavity preparation, flat-end diamond fissure bur (no. 121415, Shofu Inc, Japan) in an air turbine handpiece mounted on a modified dental surveyor ensures parallelism between the long axis of the bur and the tooth [29 ]. To ensure standardization, a periodontal probe and caliper were used to check the cavity depth and dimensions.
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7

Fluoride Release from Restorative Materials

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A total of 30 disks, 10 disks of each restorative material R1: Jen Rainbow, Jen Dent Ukraine; R2: Tetric® N-Flow, Ivoclar-Vivadent; and R3: RMGIC (Fuji II LC- GC Corporation) were fabricated in polytetrafluoroethylene mold (5 × 2 mm diameter), and were tested for F dynamics in two different media M1: artificial saliva, M2: deionized water group. Dental floss was incorporated into each disk, and a mylar strip was supported on either side by glass slabs prior to curing. The cured disk was stored at room temperature for 24 hours (Figs 4 and 5).13 (link)–15 (link)
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8

Fractured Tooth Restoration Protocol

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The preconditioned fractured fragments and remaining tooth structures were rinsed thoroughly with distilled water and acid etched with 37% phosphoric acid (N-Etch, Ivoclar Vivadent, Germany) for 15 sec, followed by rinsing with water for 15 s and gently air-dried for 5 s to keep the surface moist. The first coat of bonding agent (Tetric N-Bond, Ivoclar Vivadent, Germany) was applied over the sectioned surfaces for 10 s, and then, the second coat was applied and air thinned to remove any excess which was then light cured (850-1000 w/cm 2 , Woodpecker, London) for 20 s. The fractured fragments were proximated and re-attached using flowable composite resin (Tetric N-Flow, Ivoclar Vivadent, Germany) and cured for 20 s on the labial and palatal surfaces. After re-attachment, the specimens were placed in artificial saliva at room temperature until they were tested for fracture resistance.
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9

Evaluation of Flowable Resin Composites

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Five commercial flowable resin composites were tested in this study: AliteFlo LV (AF: Bisco, Schaumburg, IL, USA), Flow-it ALC (FI: Jeneric/Pentron, Wallingford, CT, USA), Venus flow (VF: Heraeus-Kulzer, Weihrheim, Germany), Tetric N-flow (TF: Ivoclar-Vivadent, Schaan, Liechtenstein), and Revolution Formula2 (RV: Kerr, Orange, CA, USA) (Table 1).
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10

Molar Amputation and Root Canal Preparation

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All molars were amputated 2 mm below the furcation area using a tapered diamond stone (Dentsply Maillefer, Ballaigues, Switzerland). Complete de-roofing of the pulp chamber of each molar was done using a round bur #2 (Dentsply Maillefer, Ballaigues, Switzerland) and the root canal orifices were located. 28 The canal orifices and the apical end of each root were etched with 37% phosphoric acid gel (Ivoclar Vivadent, Liechtenstein) for 30 seconds and were thoroughly rinsed and dried. Then, the adhesive (Excite F, Ivoclar Vivadent, Liechtenstein) was applied in two consecutive coats followed by photo-polymerization for 10 seconds with light curing unit (LED: Bluephase ® C5 curing-light, 500 mW/cm 2 , 430-490 nm, Ivoclar Vivadent AG FL-9494 Schaan/Liechtenstein, Austria).
Flowable resin composite (Tetric N-Flow, Ivoclar Vivadent, Liechtenstein) was used to fill the root canal orifices as well as the apical ends of the roots, then it was photo-polymerized for 40 seconds. 29
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