Rapamycin was obtained from LC Labs. (-)-OC, extracted from EVOO, was obtained from Dr. Alexios-Leandros Skaltsounis at the University of Athens Department of Pharmacology. The structure and purity (97%) of (-)-oleocanthal was determined by HPLC and 1H-NMR analysis. Acridine orange was obtained from Life Technologies. The Promokine Apoptotic/Necrotic/Healthy cell detection kit and Cerulenin were obtained from Fisher Scientific. The Sphingomyelinase Fluorometric Assay kit was obtained from Cayman Chemicals. Primary antibodies specific for cleaved caspase-3, cleaved PARP, phospho-p44/42 (Thr202/Tyr204), total p44/42, β-actin, and phospho-Rb (Ser 608) were obtained from Cell Signaling Technology. Antibodies against glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and Hsp70 were obtained from Santa Cruz Biotechnology.
Phospho rb ser 608
Manufactured by Cell Signaling Technology
Phospho-Rb (Ser 608) is a lab equipment product that detects phosphorylation of the retinoblastoma protein at serine 608. It is used in research applications to study cell cycle regulation and signaling pathways.
Automatically generated - may contain errors
Lab products found in correlation
Acridine orange, by Thermo Fisher Scientific (2 mentions)
Cerulenin, by Thermo Fisher Scientific (2 mentions)
Total p44 42, by Cell Signaling Technology (2 mentions)
β actin, by Cell Signaling Technology (2 mentions)
Promokine apoptotic necrotic healthy cell detection kit, by Thermo Fisher Scientific (2 mentions)
Sphingomyelinase fluorometric assay kit, by Cayman Chemical (2 mentions)
Antibodies against glyceraldehyde 3 phosphate dehydrogenase gapdh and hsp70, by Santa Cruz Biotechnology (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Decitabine, by Merck Group (1 mentions)
Cyclin d1, by Cell Signaling Technology (1 mentions)
Pf 04691502, by Selleck Chemicals (1 mentions)
Rapamycin, by Selleck Chemicals (1 mentions)
Selumetinib, by Selleck Chemicals (1 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
Caspase 9, by Cell Signaling Technology (1 mentions)
Imatinib, by Selleck Chemicals (1 mentions)
Dacomitinib, by Selleck Chemicals (1 mentions)
β actin antibody, by Merck Group (1 mentions)
Phospho akt ser473, by Cell Signaling Technology (1 mentions)
Sunitinib, by Selleck Chemicals (1 mentions)
3 protocols using phospho rb ser 608
1
Rapamycin and Oleocanthal Synergistic Effects
2
Rapamycin and Oleocanthal Synergistic Effects
3
Comprehensive Antibody Characterization Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antibody for total RB was purchased from BD Biosciences (San Jose, CA, clone C245, catalog #554136). Antibodies purchased from Cell Signaling Technology (Beverly, MA) included: total RB (clone 4H1, Catalog #9309), phospho-RB (Ser608) (#2181), phospho-AKT (Ser473) (#4060), cyclin D1 (#9309), PARP-1 (#9542), caspase 3 (#9665), caspase 9 (#9502), and phospho-p70 S6K (Thr389, #9205). β-actin antibody was purchased from Sigma Aldrich (St. Louis, MO, catalog #A1978).
Laboratory-grade palbociclib was generously provided by Pfizer, Inc. (New York, NY) and purchased from Selleck Chemicals (Boston, MA). Everolimus and pemetrexed were purchased from LC chemical (Woburn, MA). Decitabine was purchased from Sigma (St. Louis, MO). Selumetinib, flavopiridol, sunitinib, AZD5438, ponatinib, AZD4547, erlotinib, pazopanib, imatinib, PF-04691502, dacomitinib and rapamycin were purchased from Selleck Chemicals (Boston, MA). Depsipeptide was obtained from the NCI Repository in the Developmental Therapeutics Program (NSC #309132, Bethesda, MD). Stock solutions for all the agents were prepared in 100% DMSO (Sigma, St. Louis, MO) at 10mM concentrations and stored at -20°C. Stock solutions were diluted in fresh RPMI 1640 media prior to each experiment.
Laboratory-grade palbociclib was generously provided by Pfizer, Inc. (New York, NY) and purchased from Selleck Chemicals (Boston, MA). Everolimus and pemetrexed were purchased from LC chemical (Woburn, MA). Decitabine was purchased from Sigma (St. Louis, MO). Selumetinib, flavopiridol, sunitinib, AZD5438, ponatinib, AZD4547, erlotinib, pazopanib, imatinib, PF-04691502, dacomitinib and rapamycin were purchased from Selleck Chemicals (Boston, MA). Depsipeptide was obtained from the NCI Repository in the Developmental Therapeutics Program (NSC #309132, Bethesda, MD). Stock solutions for all the agents were prepared in 100% DMSO (Sigma, St. Louis, MO) at 10mM concentrations and stored at -20°C. Stock solutions were diluted in fresh RPMI 1640 media prior to each experiment.
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