Isolera

Manufactured by Biotage

Sourced in Sweden

Isolera is a laboratory equipment product designed for automated flash chromatography. It facilitates the separation and purification of chemical compounds. The core function of Isolera is to provide a controlled and efficient method for conducting flash chromatography experiments.

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Lab products found in correlation

Methanol d4, by Cambridge Isotopes (1 mentions) Kojic acid, by Merck Group (1 mentions) Mushroom tyrosinase, by Merck Group (1 mentions) L tyrosine, by Merck Group (1 mentions) Formic acid, by Merck Group (1 mentions) Spectrospin 300 mhz spectrometer, by Bruker (1 mentions) Silica gel, by Merck Group (1 mentions) Milli q water system, by Merck Group (1 mentions) Sephadex lh 20, by Cytiva (1 mentions) Agilent chemstation software, by Agilent Technologies (1 mentions) 60f254 silica gel plate, by Merck Group (1 mentions) 6130 single quadrupole mass spectrometer, by Agilent Technologies (1 mentions) 1100 hplc system, by Agilent Technologies (1 mentions) Photodiode array detector, by Agilent Technologies (1 mentions) Spectrum 100 ftir spectrometer, by PerkinElmer (1 mentions) Avance 3, by Bruker (1 mentions) Mercury vx 300, by Agilent Technologies (1 mentions) Mercury plus 300, by Agilent Technologies (1 mentions) Mercury vx400, by Agilent Technologies (1 mentions) Avance 500 spectrometer, by Bruker (1 mentions)

Spelling variants of this product

Isolera One (36 citations) Isolera One flash purification system (9 citations) Isolera FLASH purification system (7 citations) Isolera SNAP 10 g (4 citations) Isolera flash chromatography system (3 citations) Isolera chromatography system (3 citations) Isolera SNAP (2 citations) Isolera apparatus (2 citations) Isolera 1 System (2 citations) Isolera-one flash chromatography (2 citations)

21 protocols using isolera

Synthesis of Furan-Containing Piperazine Derivative

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Example 6

Trimethyl aluminum (2M in toluene, 0.38 mmol) was added to a solution of benzyl amine (0.37 mmol) in anhydrous DCM (1 mL) at RT and the resulting mixture was stirred for 15 min. A solution of (S)-tert-butyl 4-(3-(furan-2-carboxamido)-4-methoxy-4-oxobutyl)piperazine-1-carboxylate 30 (0.12 mmol) in anhydrous DCM was added at RT for 3.5 h then NaOH (aq) (1M, 1 mL). The product was extracted with DCM and purification by Biotage™ Isolera (EtOAc in hexane) to obtain the desired product 32 (64%). ¹H NMR (CDCl3) δ 8.64 (d, J=6.53 Hz, 1H), 7.46 (d, J=1.00 Hz, 1H), 7.22-7.40 (m, 6H), 7.12 (d, J=3.51 Hz, 1H), 6.52 (dd, J=1.76, 3.51 Hz, 1H), 4.65-4.74 (m, 1H), 4.47-4.56 (m, 1H), 4.38-4.46 (m, 1H), 3.38 (br. s., 4H), 2.56-2.67 (m, 1H), 2.33-2.51 (m, 3H), 2.14-2.31 (m, 3H), 1.91 (ddt, J=3.73, 7.40, 14.74 Hz, 1H), 1.47 (s, 9H).

US10716791B2. Inhibitors of peptidyl arginine deiminase (PAD) enzymes and uses thereof (2020-07-21). UNIVERSITY HEALTH NETWORK [CA]. Inventors: Lakshmi P. Kotra [CA], Ewa Wasilewski [CA].

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Purification and NMR Analysis of Compounds

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Medium pressure liquid chromatography (MPLC) was conducted using a Biotage Isolera (Biotage AB, Uppsala, Sweden). One system equipped with a high performance flash chromatography (HPFC) pump, a variable dual-wavelength detector, and a collector. NMR spectra were acquired using a Bruker SPECTROSPIN 300 MHz spectrometer (Bruker Corporation, Billerica, MA, USA). Methanol-d₄, an NMR solvent, was purchased from Cambridge Isotope Laboratories, Inc. Acetonitrile (ACN), water, and methanol (MeOH) of chromatographic grade were purchased from ThermoFisher Scientific Korea Ltd. (Seoul, Republic of Korea). L-tyrosine, mushroom tyrosinase, kojic acid, and formic acid were purchased from Sigma–Aldrich Co (St. Louis, MO, USA).

Oh K.E., Shin H., Lee M.K., Park B, & Lee K.Y. (2021). Characterization and Optimization of the Tyrosinase Inhibitory Activity of Vitis amurensis Root Using LC-Q-TOF-MS Coupled with a Bioassay and Response Surface Methodology. Molecules, 26(2), 446.

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Purification of Fluorophenyl Carbamate

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The compound tert-butyl (2-amino-4-fluorophenyl)carbamate (36) was purified by chromatography using Biotage^® Isolera TM (SNAP ultra 10; with hexane/ethyl acetate 6:4 as eluents in an isocratic method; 36 mL/min flux and 254 nm wavelength detection), leading to the formation of the pure product, as a yellow solid at a yield of 86%. ¹H NMR (600 mHz, DMSO- d₆) δ 8.41 (s, 1H); 7.19 (m, 1H); 6.65 (dd; J = 6.9, 1.5 Hz; 2H); 4.77 (s, 2H); 1.46 (s, 9H).

Lopes J.R., Prokopczyk I.M., Gerlack M., Man Chin C, & Santos J.L. (2021). Design and Synthesis of Hybrid Compounds as Epigenetic Modifiers. Pharmaceuticals, 14(12), 1308.

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Synthesis and Purification of Carbamate Compound

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Compound tert-butyl (4-fluoro-2-(4-iodobenzamido)phenyl)carbamate (37) was purified by chromatography using the Biotage^® Isolera TM (SNAP ultra 10; with hexane/ethyl acetate (7:3) as eluents in an isocratic method; 36 mL/min flux and 254 nm wavelength detection), leading to the formation of the pure product, as a light brown solid at yield of 65%. ¹H NMR (600 mHz, DMSO-d₆) δ 9.82 (s, 1H); 8.80 (s, 1H); 7.93 (d; J = 8.5 Hz; 2H); 7.74 (d; J = 8.4 Hz; 2H); 7.54 (dd; J = 11.2, 2.9 Hz; 1H); 7.41 (dd; J = 8.8, 6.3 Hz; 1H); 6.95 (ddd, J = 11.1, 8.4, 3.0 Hz, 1H); 1.44 (s, 9H).

Lopes J.R., Prokopczyk I.M., Gerlack M., Man Chin C, & Santos J.L. (2021). Design and Synthesis of Hybrid Compounds as Epigenetic Modifiers. Pharmaceuticals, 14(12), 1308.

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NMR Spectroscopy and Flash Chromatography

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¹H-NMR spectra were recorded using an FT-spectrometer operating at 300 MHz while ¹³C-NMR were recorded at 75.43 MHz. Chemical shifts are reported in ppm relative to the residual solvent (CHCl₃) as an internal standard. Signal multiplicity is designed according to the following abbreviations: s = singlet, d = doublet, dd = doublet of doublets, t = triplet, m = multiplet, and bs = broad singlet. Purifications were performed by flash chromatography using silica gel (particle size 40–63 μm, Merck) on Isolera^TM (Biotage, Uppsala, Sweden) apparatus.

Artasensi A., Salina E., Fumagalli L, & Regazzoni L. (2023). A Novel Chromatographic Method to Assess the Binding Ability towards Dicarbonyls. Molecules, 28(14), 5341.

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Synthesis and Characterization of Carnosine Derivatives

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Water, HPLC grade (18 MΩ), was purified with a Milli-Q water system (Millipore, Milan, Italy). l-carnosine was kindly provided by Flamma s.p.a. (Chignolo D’isola, Bergamo, Italy). TRH, CHP, His-Pro, solvents at HPLC grade and all other chemicals were purchased from Merck KGaA, Darmstadt, Germany. ¹H-NMR spectra were recorded using a FT-spectrometer operating at 300 MHz while ¹³C-NMR at 75.43 MHz. Chemical shifts are reported in ppm relative to the residual solvent (CHCl₃, MeOH, or DMSO) as an internal standard. Signal multiplicity is designed according to the following abbreviations: s = singlet, d = doublet, dd = doublet of doublets, t = triplet, m = multiplet, br s = broad singlet and br t = broad triplet. Purifications were performed by flash chromatography using silica gel (particle size 40–63 μm, Merck) on Isolera^TM (Biotage, Uppsala, Sweden) apparatus.

Regazzoni L., Fumagalli L., Artasensi A., Gervasoni S., Gilardoni E., Mazzolari A., Aldini G, & Vistoli G. (2022). Cyclo(His-Pro) Exerts Protective Carbonyl Quenching Effects through Its Open Histidine Containing Dipeptides. Nutrients, 14(9), 1775.

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Flash Column Chromatography Separations

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Flash column chromatography separations were performed by using silica gel (80 mesh and 300 mesh; Fuji silysia Co. (Aichi, Japan)) or Biotage Isolera^TM (Uppsala, Sweden) equipped with Biotage SNAP Ultra Silica Cartridges (10 g, 25 g, 50 g, 100 g, and 340 g) (Uppsala, Sweden) or Biotage Sfär Silica HC D Cartridges (10 g, 25 g, 100 g, 200 g, and 350 g) (Uppsala, Sweden). The quantity of silica gel was typically 100 to 200 times the weight of the crude sample. Cytiva Sephadex LH-20 (Tokyo, Japan) was used for size-exclusion chromatography. Solvent systems for chromatography are specified as v/v ratios.

Hamajima S., Komura N., Tanaka H.N., Imamura A., Ishida H., Ichiyanagi T, & Ando H. (2022). Investigation of the Protection of the C4 Hydroxyl Group in Macrobicyclic Kdo Donors. Molecules, 28(1), 102.

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Purification of Compound 10 by Chromatography

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Compound 4-(3,5-dimethylisoxazol-4-yl)--(4-fluoro-2-nitrophenyl)benzamide (10) was purified by chromatography using the Biotage^® Isolera TM (SNAP ultra 10; with hexane/ethyl acetate 6:4 as eluents in an isocratic method; 36 mL/min flux and 254 nm wavelength detection), leading to the formation of the pure product, as a brown solid at yields of 26%. ¹H NMR (600 mHz, DMSO-d₆) 8.77 (dd, J = 4.4, 1.4 Hz, 1H); 8.54 (dd, J = 8.4, 1.4 Hz, 1H); 8.02 (m, 2H); 7.52 (m, 3H); 2.44 (s, 3H); 2.25 (s, 3H); ¹³C (150 mHz, DMSO- d₆) δ 134.68, 151.48, 143.37, 121.31, 129.13, 124.90, 167.04, 134.49, 129.29, 130.10, 129.67, 115.31, 165.89, 11.49, 158.07, 10.55; MS/ESI m/z calculated for C₁₈H₁₄FN₃O₄: 356.10, found: [M+H]+ 357.11; mp: 134–140 °C.

Lopes J.R., Prokopczyk I.M., Gerlack M., Man Chin C, & Santos J.L. (2021). Design and Synthesis of Hybrid Compounds as Epigenetic Modifiers. Pharmaceuticals, 14(12), 1308.

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Purification of Tert-Butyl Carbamate Compound

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Compound tert-butyl (2-amino-4-(3,5-dimethylisoxazol-4-yl)phenyl)carbamate (21) was purified by chromatography using Biotage^® Isolera TM (SNAP ultra 10; with hexane/ethyl acetate 7:3 as eluents in an isocratic method; 36 mL/min flux and 254 nm wavelength detection) leading to the formation of the pure product, a light brown solid at yield of 45%. ¹H NMR (600 mHz, DMSO- d₆) δ 8.38 (s, 1H); 7.28 (d, 1H); 6.68 (d, 1H); 6.51 (dd, 1H); 4.97 (s, 2H); 2.37 (s, 3H); 2.19 (s, 3H); 1.47 (s, 9H); ¹³C (150 mHz, DMSO- d₆) δ 11.35, 158.11, 10.58, 152.47, 126.15, 123.17, 115.85, 116.07, 116.84, 124.56, 141.30, 164.44, 28.17, 78.85.

Lopes J.R., Prokopczyk I.M., Gerlack M., Man Chin C, & Santos J.L. (2021). Design and Synthesis of Hybrid Compounds as Epigenetic Modifiers. Pharmaceuticals, 14(12), 1308.

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Glycan Purification by Flash Chromatography

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Example 14

In one example, the glycan polymer preparation was concentrated on a rotatory evaporator to approximately 50 Brix as measured by a Brix refractometer. The resulting syrup (200 mg) was loaded onto a Teledyne ISCO RediSep Rf Gold Amine column (11 grams stationary phase) using a luer-tip syringe. Other similar columns such as the Biotage SNAP KP-NH Cartridges may also be used. The sample was purified on a Biotage Isolera equipped with an ELSD detector using a 20/80 to 50/50 (v/v) deionized water/ACN mobile phase gradient over 55 column volumes. Other flash chromatography systems such as the Teledyne ISCO Rf may also be used. The flow rate was set in accordance with the manufacturer's specifications for the column and system. After the monomer fraction completely eluted at ˜20 column volumes, the mobile phase was set to 100% water until the remainder of the glycan eluted and was collected. The non-monomer containing fractions were concentrated by rotary evaporation to afford the de-monomerized product.

US11697692B2. Methods of producing glycan polymers (2023-07-11). DSM Nutritional Products, LLC [US]. Inventors: John M. Geremia [US], Christopher Matthew Liu [US], Anastasia V. Lioubomirov [US].

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