Sb203580 s8307

The a-Modified minimal essential medium was purchased from Thermo Scientific (Waltham, MA, USA). Polystyrene culture dishes were obtained from Costar (Corning, NY, USA), and fetal bovine serum was purchased from Gibco Life Technologies (Waltham, MA, USA). Anti-GPR120 (sc48203) and anti-GPR40 (sc32905) were purchased from Santa Cruz (Dallas, TX, USA). Anti-p62 (ab56416), anti-mTOR (ab32028), anti-p-mTOR (ab109268), anti-S6K1 (ab32529), anti-p-S6k1 (ab5231), anti-Atg7 (ab133528) and anti-LC3B (ab51520) were purchased from Abcam (Cambridge, MA, USA).TUG-891 (4-[(4-Fluoro-4'-methyl[1,1'-biphenyl]-2-yl)methoxy]-benzenepropanoic acid) (4601-50) and U0126 (1144/25) were purchased from R&D Systems (Minneapolis, MN, USA). LY294002 (L9908), SB203580 (S8307) and BafA1 (B1793) were purchased from Sigma Chemical (St. Louis, MO, USA). Penicillin–streptomycin was purchased from Solarbio (Beijing, China). The total RNA extraction kit was purchased from OMEGA (Norcross, GA, USA). The PrimeScript RT reagent kit and SYBR Premix Ex Taq were purchased from TaKaRa (Dalian, China). Other reagents were of the highest commercial grade and purchased from Sigma Chemical.

Antibodies and regents used in this experiment are all obtained commercially: Anti-Drosha antibody (ab12286), Anti-DGCR8 (ab191875) was purchased from Abcam (Cambridge, UK). Anti-TH antibody (P8984), anti-β-actin antibody (A5441), DAPI (D9542), 6-Hydroxydopamine hydrochloride (H4381), and SB203580 (S8307) were purchased from Sigma (St. Louis, MO, USA). Anti-phospho S/P (2325), Anti-phospho p38 (9211), anti p38 MAPK (8690), and anti-cleaved caspase3 (9664) were from Cell Signaling technology (Cambridge, MA, USA). Anti Flag (20543-1-AP) was purchased from Proteintech (Wuhan, China). Calpeptin (S7396) and MG132 (S2619) were purchased from selleck (Houston, USA). A TUNEL staining kit (QIA39) was purchased from Calbiochem (Boston, MA, USA). Vectastain ABC kit (PK-6101) was purchased from Vector Laboratories (Burlingame, CA, USA). DAB staning kit (CW0125) was purchased from CWBIO (Beijing, China). Thiazolyl blue (M8180) was purchased from Solarbio (Beijing, China).