Mem vitamins solution

Manufactured by Thermo Fisher Scientific

Sourced in United States

MEM vitamins solution is a ready-to-use solution containing essential vitamins required for cell culture media. It is designed to supplement Minimum Essential Medium (MEM) to provide a balanced vitamin composition to support cell growth and maintenance.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

MEM vitamin solution (106 citations) MEM vitamins (66 citations) Vitamin solution (20 citations) MEM Vitamin solution 100X (5 citations)

7 protocols using mem vitamins solution

Extracellular Proteome of C. pseudotuberculosis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Corynebacterium pseudotuberculosis biovar equi strain 258 was isolated from a horse in Belgium; this strain was cultivated under routine conditions in brain–heart infusion broth (BHI-HiMedia Laboratories Pvt. Ltd., India) at 37°C. When necessary, 1.5% agar was added to the medium for solid culture. For extracellular proteomic analyses, 258_equi was grown in a chemically defined medium (CDM) [(Na₂HPO₄_7H₂O (12.93 g/L), KH₂PO₄ (2.55 g/L), NH₄Cl (1 g/L), MgSO₄_7H₂O (0.20 g/L), CaCl₂ (0.02 g/L), and 0.05% (v/v) Tween 80] with 4% (v/v) MEM Vitamins Solution (Invitrogen, Gaithersburg, MD, USA), 1% (v/v) MEM Amino Acids Solution (Invitrogen), 1% (v/v) MEM Non-Essential Amino Acids Solution (Invitrogen), and 1.2% (w/v) glucose at 37°C (Moura-Costa et al., 2002 ).

Silva W.M., Carvalho R.D., Dorella F.A., Folador E.L., Souza G.H., Pimenta A.M., Figueiredo H.C., Le Loir Y., Silva A, & Azevedo V. (2017). Quantitative Proteomic Analysis Reveals Changes in the Benchmark Corynebacterium pseudotuberculosis Biovar Equi Exoproteome after Passage in a Murine Host. Frontiers in Cellular and Infection Microbiology, 7, 325.

+ Open protocol

+ Expand

Cultivation of C. pseudotuberculosis ovis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The C. pseudotuberculosis biovar ovis strain 1002 (1002_ovis) was isolated from a goat in Brazil; this strain was cultivated under standard conditions in brain–heart infusion broth (BHI-HiMedia Laboratories Pvt. Ltd., India) at 37 °C. When necessary, 1.5% of agar was added to the medium for a solid culture. For extracellular proteomic analyses, 1002_ovis was grown in a chemically defined medium (CDM) [(Na₂HPO₄_7H₂O (12.93 g/L), KH₂PO4 (2.55 g/L), NH₄Cl (1 g/L), MgSO₄_7H₂O (0.20 g/L), CaCl₂ (0.02 g/L) and 0.05% (v/v) Tween 80], 4% (v/v) MEM Vitamins Solution (Invitrogen, Gaithersburg, MD, USA), 1% (v/v) MEM Amino Acids Solution (Invitrogen), 1% (v/v) MEM Non-Essential Amino Acids Solution (Invitrogen), and 1.2% (w/v) glucose at 37 °C [22 ].

Silva W.M., Dorella F.A., Soares S.C., Souza G.H., Castro T.L., Seyffert N., Figueiredo H., Miyoshi A., Le Loir Y., Silva A, & Azevedo V. (2017). A shift in the virulence potential of Corynebacterium pseudotuberculosis biovar ovis after passage in a murine host demonstrated through comparative proteomics. BMC Microbiology, 17, 55.

+ Open protocol

+ Expand

Optimized Culture Media for Cell Growth

Check if the same lab product or an alternative is used in the 5 most similar protocols

Earle’s Balanced Salt Solution (EBSS; Gibco #240010) was supplemented with 0.4 mM L-serine, 0.2 mM L-methionine, 0.2 mM L-histidine monohydrochloride, 0.8 mM L-threonine, 0.399 mM L-arginine monohydrochloride, 0.8 mM L-lysine monohydrochloride, 0.4 mM L-phenylalanine, 0.2 mM L-cystine hydrochloride, 0.4 mM glycine, 0.078 mM L-tryptophan, 0.248 µM ferric nitrate nonahydrate and filtered. The solution was further supplemented with 1× MEM vitamins solution (Gibco, containing calcium pantothenate, choline chloride, folic acid, inositol, niacinamide, pyridoxine HCl, riboflavin, thiamine HCl), 1 mM sodium pyruvate, 2 mM L-Glutamine, 10% dialysed foetal bovine serum (Gibco), and 50 IU/mL penicillin/streptomycin. A “100×” stock of BCAA (Sigma-Aldrich) was prepared containing 79.9 mM leucine, isoleucine and valine and used to supplement the media as required.

Ibrahim S.L., Abed M.N., Mohamed G., Price J.C., Abdullah M.I, & Richardson A. (2022). Inhibition of branched-chain alpha-keto acid dehydrogenase kinase augments the sensitivity of ovarian and breast cancer cells to paclitaxel. British Journal of Cancer, 128(5), 896-906.

+ Open protocol

+ Expand

Cytotoxicity Assay of TTR Compounds in SH-SY5Y Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human neuroblastoma cell line SH-SY5Y was obtained from the European Collection of Cell Cultures (Centre for Applied Microbiology and Research). SH-SY5Y cells were cultured according to a previous report [79 (link)] with minor modifications. The cells were grown in MEM and GlutaMax medium (Gibco) and supplemented with 10% (v/v) fetal bovine serum (Gibco), 100 units/mL penicillin, 100 μg/mL streptomycin (Gibco), and 1% non-essential amino acid solution (Gibco). Cultures were maintained in an incubator at 37°C with a humidified atmosphere of 5% CO₂. Freshly purified TTR (15 μM) in MEM was sterile-filtrated and pre-incubated with TBBPA, tafamidis, or diflunisal (15 μM each) for one hour before being added to the SH-SY5Y cells. The protein-compound solutions were supplemented with 100 units/mL penicillin, 100 μg/mL streptomycin (Gibco), 1% non-essential amino acid solution (Gibco), 2 mM L-glutamine (Gibco), 1% MEM vitamins solution (Gibco), and 1% MEM amino acids solution (Gibco) and incubated with the cells for 48 h at 37°C in a humidified atmosphere of 5% CO₂. Cytotoxicity was measured using a resazurin reduction test, and cell viability was detected by fluorescence measurement using a TECAN Safire plate reader with excitation at 535 nm and emission at 595 nm. All experiments were performed in triplicate.

Iakovleva I., Begum A., Brännström K., Wijsekera A., Nilsson L., Zhang J., Andersson P.L., Sauer-Eriksson A.E, & Olofsson A. (2016). Tetrabromobisphenol A Is an Efficient Stabilizer of the Transthyretin Tetramer. PLoS ONE, 11(4), e0153529.

+ Open protocol

+ Expand

Granulocyte-Bacteria Interaction Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

The isolated granulocytes were incubated with mCherry-E. coli strains (Table S1) at a ratio of 200 bacteria per 1 granulocyte, at 37°C in a water bath. Incubation was performed in hemolysis tubes in RPMI 1640 medium, supplemented with 10% heat-inactivated fetal bovine serum (Eurobio), 1% non-essential amino acids (Gibco), L-glutamine (200 mM, Gibco), sodium pyruvate solution (100 mM, Gibco) and MEM vitamins solution (100X, Gibco). Samples from shorter timepoints were maintained on ice until processing.

Chollet S., Hernandez Padilla A.C., Daix T., Gaschet M., François B., Piguet C., Gachard N., Da Re S., Jeannet R, & Ploy M.C. (2024). Phagosomal granulocytic ROS in septic patients induce the bacterial SOS response. iScience, 27(6), 109825.

+ Open protocol

+ Expand

Bone Marrow-Derived Macrophage Isolation and Stimulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bone marrow-derived macrophages (BMDMs) were isolated from C57BL/6J, Ifnar1^–/– and Setdb2^GT/GT mice and cultured in RPMI medium containing 10% FBS, penicillin-streptomycin-glutamine (Life Tech #10378-016) and 50ng/ml recombinant mouse macrophage colony stimulating factor (eBioscience #34-8983-85). BMDMs were stimulated with PAM3CSK4 (PAM3) (500ng/ml, Invivogen tlrl-pms), poly(I:C) (6μg/ml, Invivogen tlrl-pic), LPS (20ng/ml, Salmonella enterica serotype Minnesota, Sigma #L4641), mouse IFNβ (1000 IU/ml, PBL Interferon Source #12400-1), mouse IFNγ (100ng/ml, Peprotech #315-05) or IFNλ2 (100ng/ml, Biomedica 4635-ML-025). For in vitro infections with influenza virus A/PR/8/34, cells were grown in OPTI-MEM medium (Life Tech, #31985-070) containing 4% BSA (Sigma, #A7979), 1x MEM vitamins solution (Life Tech, #11120-037) and 1μg/ml TPCK trypsin (Sigma, #T8802). To block IFNAR1, we pre-treated BMDMs with 20μg/ml of IFNAR1-specific antibody (clone MAR1-5A3, BioXCell #BE0241) ^{48 (link)} or with mouse IgG1 isotype control (clone MOPC-21, BioXCell #BE0083) 18 hours prior to poly(I:C) stimulation and added fresh antibody upon stimulation.

Schliehe C., Flynn E.K., Vilagos B., Richson U., Swaminanthan S., Bosnjak B., Bauer L., Kandasamy R.K., Griesshammer I.M., Kosack L., Schmitz F., Litvak V., Sissons J., Lercher A., Bhattacharya A., Khamina K., Trivett A.L., Tessarollo L., Mesteri I., Hladik A., Merkler D., Kubicek S., Knapp S., Epstein M.M., Symer D.E., Aderem A, & Bergthaler A. (2014). The methyltransferase Setdb2 mediates virus-induced susceptibility to bacterial superinfection. Nature immunology, 16(1), 67-74.

+ Open protocol

+ Expand

Bone Marrow-Derived Macrophage Isolation and Stimulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!