The anti-mouse IgG conjugated with HRP was purchased from Invitrogen (Thermo Fisher Scientific, Waltham, MA, USA). All the antibodies for the Western blot experiments were obtained from Cell Signaling Technology® (Danvers, MA, USA.). All the other reagents and chemicals were purchased from Merck® (Darmstadt, Germany). The cell culture wares, including plates and flasks (polystyrene coated), were obtained from Nunc Cell Culture® (Thermo Fisher Scientific, Waltham, MA, USA).
Purpald
Purpald is a laboratory reagent used for the detection and quantification of aldehydes. It is a colorimetric assay that produces a purple color when reacted with aldehydes, allowing for spectrophotometric measurement and analysis.
Lab products found in correlation
6 protocols using purpald
In Vitro Cell Cytotoxicity Evaluation
The anti-mouse IgG conjugated with HRP was purchased from Invitrogen (Thermo Fisher Scientific, Waltham, MA, USA). All the antibodies for the Western blot experiments were obtained from Cell Signaling Technology® (Danvers, MA, USA.). All the other reagents and chemicals were purchased from Merck® (Darmstadt, Germany). The cell culture wares, including plates and flasks (polystyrene coated), were obtained from Nunc Cell Culture® (Thermo Fisher Scientific, Waltham, MA, USA).
Antioxidant Assays for α-Cypermethrin and Curcumin
Quantification of Bacterial Lipopolysaccharide
Catalase Activity Assay Protocol
Preparation and Quantification of Salmonella Outer Membrane Vesicles
Enzymatic Glycerol Oxidation Assay
with glycerol (205 mM) in 100 mM potassium phosphate pH 7.5 containing
2 mM 1,4-dithiothreitol and 1014 U catalase (Micrococcus lysodeikticus, Sigma–Aldrich), at 25 °C and 50 rpm (Innova 40 incubator
shaker; 2 mL reactions in 20 mL vials). After 68 h of incubation,
200 μL of the reactions were mixed with 200 μL of Purpald
(Sigma–Aldrich, 5 mg/mL in 0.5 N NaOH). Next, samples were
incubated at 30 °C and 700 rpm for 30 min (Eppendorf ThermoMixer
C) and subsequently mixed with 600 μL of water. Finally, 50
μL of the resulting solutions were diluted with 950 μL
of water to record their absorbance spectrum. In parallel, the same
procedure was performed for control reactions without F101S PcAOX,
except that the last dilution (1:20) was not performed before measuring.
Similarly, 200 μL of 1 mM glyceraldehyde, glycerol, and glyceric
acid were reacted with 200 μL of Purpald. Subsequently, these
standard solutions were diluted with water (final concentration =
0.2 mM, 1 mL) just prior to recording their absorbance spectrum.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!