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Cd40l bv605

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CD40L BV605 is a fluorophore-conjugated antibody that binds to the CD40 ligand protein. CD40 ligand is a type II transmembrane protein that plays a crucial role in the activation of B cells, T cells, and antigen-presenting cells. The BV605 fluorophore allows for detection and analysis of CD40 ligand expression on cells using flow cytometry.

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Lab products found in correlation

Il 2 bv711, by BD (3 mentions) Il 21 pe, by Thermo Fisher Scientific (3 mentions) Ifn γ pe cy7, by BD (3 mentions) Cxcr5alexa647, by BD (2 mentions) Pd 1 bv650, by BD (2 mentions) Live dead fixable blue dead cell stain kit, by Thermo Fisher Scientific (2 mentions) Tnf α fitc, by BD (2 mentions) Cd4 apc cy7, by BD (2 mentions) Perforin pe dazzle 594, by BioLegend (2 mentions) Cd69 bv650, by BD (2 mentions) Cd27 bv480, by BD (2 mentions) Cd45ro pe cy5, by Beckman Coulter (2 mentions) Cd8 percp, by BioLegend (2 mentions) Pd 1 bv421, by BioLegend (2 mentions) Cd3 percp cy5, by BioLegend (1 mentions) Pd 1 pe, by BioLegend (1 mentions) Ccr7 pecy7 3d12, by BD (1 mentions) Cd138 percpcy5, by BioLegend (1 mentions) Ber act35, by BioLegend (1 mentions) Cd19 bv650, by BioLegend (1 mentions)

5 protocols using cd40l bv605

1

Comprehensive Immune Cell Phenotyping

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Directly conjugated antibodies were purchased from BD Biosciences (San Jose, CA, USA): CCR7-PECy7 (3D12), CD3-PerCPCy5.5 and CD3-V500 (UCHT1), Foxp3-AF488 (259D/C7), CD25-AF700 (M-A251), CD4-APCH7 (RPA-T4), CXCR5-AF647 (RF8.B2), CD45RA-PECF594 (HI100), CD19-APCCy7 (SJ25C1), IgM-FITC (G20-127), IgD-PECF594 (IA6-2), CD38-V450 (HB7), CD138-PerCPCy5.5 (MI15), CD21-APC (B-LY4), CD27-PE (M-T271), IgG-BV605 (G18-145) or BioLegend (San Diego, CA, USA): PD1-PE (EH12.2H7), CD19-BV650 (HIB19), CD57-PB (HCD57), ICOS-FITC and ICOS-PerCPCy5.5 (C398.4A), OX40-PECy7 (BER-ACT35), CD40L-BV605 (24–31).
Colineau L., Rouers A., Yamamoto T., Xu Y., Urrutia A., Pham H.P., Cardinaud S., Samri A., Dorgham K., Coulon P.G., Cheynier R., Hosmalin A., Oksenhendler E., Six A., Kelleher A.D., Zaunders J., Koup R.A., Autran B., Moris A, & Graff-Dubois S. (2015). HIV-Infected Spleens Present Altered Follicular Helper T Cell (Tfh) Subsets and Skewed B Cell Maturation. PLoS ONE, 10(10), e0140978.
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2

Flow Cytometry of Immune Cell Subsets

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The following fluorochrome conjugated antihuman monoclonal antibodies (MoAbs) were used for flow cytometry studies: ICOSBV421, ICOSAlexa488, CD40LBV605, CD69BV650, HLA-DRFITC, CD38APCCy7, and TNF-αAPCCy7 from BioLegend (San Diego, CA); CD3BUV395, CD4PerCPCy5.5, CD8Alexa-Fluor700, CCR7PECF594, IL-2BV711, CXCR5Alexa647, IFNγPE-Cy7, PD1BV650, CD45ROAPCH7, CD21PECy5, CD27PerCPCy5.5, IgDFITC, CD10PECy7, and CD20Alexa700 from BD Bioscience (San Jose, CA); IL-21PE, CD27PECy5, and IL-17Alexa488 from e-Biosciences (San Diego, CA); and CD45ROPE-TexasRed from Beckman Coulter (Fullerton, CA). Live/Dead Fixable Aqua Dead Cell Stain Kit and CellTrace Violet Cell Proliferation Kit were from ThermoFisher (Boston, MA). Recombinant human IL-21 (Cat #8879-IL-010) from R&D systems, purified antihuman IL-2 (Cat #3440-ON-500) and antihuman-IL-21 (Cat #MT216G/21.3m) from Mabtech, and antihumanTNF-α (Cat #502922) from BioLegend were used. Samples were acquired on a BD LSRFortessa (BD Biosciences, CA) flow cytometer and analyzed by FlowJo V10 (TreeStar, Inc).
Pallikkuth S., de Armas L.R., Rinaldi S., George V.K., Pan L., Arheart K.L., Pahwa R, & Pahwa S. (2019). Dysfunctional peripheral T follicular helper cells dominate in people with impaired influenza vaccine responses: Results from the FLORAH study. PLoS Biology, 17(5), e3000257.
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3

Multiparametric Flow Cytometry Immunophenotyping

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Staining was performed to determine cell viability (Flexible Viability Dye eFluor™ 780; ThermoFisher) and expression of extracellular/intracellular markers using the following fluorescently-labelled antibodies against human proteins: CD4 (FITC Mab OKT4; ThermoFisher), CD8 (BV711/clone SK1, BioLegend), CD40L (BV605; BioLegend), CD86 (PE/Cy7; BioLegend), NKG2D (PE; BioLegend), CD25 (PE/Cy7; BioLegend, CD127 (eFluor450/eBioRDR5; ThermoFisher) within different cell populations. For intracellular staining Brefeldin (3 µg/mL)(Life Technologies) was added to cultures four hours prior to flow cytometry and intracellular staining for Ki67 (Alexa Fluor 488; BioLegend) within CD4+/CD8+ T cells and iMoDC populations and staining for intracellular IFN-γ (PE clone B27; BioLegend). Staining for T regulatory (Treg) cells was performed using anti-Foxp3 (PE conjugate; BioLegend) within CD4+/CD127low/CD25+ T cells following fixation and permeabilization (Foxp3 transcription factor fixation buffer; ThermoFisher).
Agrez M., Rzepecka J., Turner D., Knox G., Chandler C., Howard C.B., Fletcher N., Thurecht K., Parker S., Gooding H, & Gallagher L. (2022). A novel lipidic peptide with potential to promote balanced effector-regulatory T cell responses. Scientific Reports, 12, 11185.
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4

Multiparameter Flow Cytometry Analysis

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The following fluorochrome-conjugated anti-human mAb were used for flow cytometry studies: LAG3 BV650, TIGIT PE-Cy7, CTLA4 PE, CD25 PE-Dazzle 594, TIM3 PerCP-Cy5.5, CD19 Alexa Fluor 700, CCR4 BV421, ICOS PE-Cy7, CCR5 PE-Dazzle 594, HLA-DR PE, CCR7 FITC, CD38 BV711, PD-L1 BV711, CCR6 Alexa Fluor 700, PD-1 BV421, CD40L BV605, perforin PE-Dazzle 594, and CD8 PerCP from BioLegend (San Diego, CA); CD3 BUV496, CD4 APC-Cy7, CD4 APC-H7, CD127 BV605, Ki-67 PerCP-Cy5.5, PD-1 BV650, CXCR3 BV605, CD69 BV650, IL-2 BV711, CXCR5 Alexa Fluor 647, IFN-γ PE-Cy7, TNF-α FITC, granzyme B Alexa Fluor 700 and CD27 BV480 from BD Biosciences (San Jose, CA); IL-21 PE from eBioscience, (San Diego, CA); and CD45RO PE-Cy5.5 from Beckman Coulter (Fullerton, CA). LIVE/DEAD Fixable Blue Dead Cell Stain Kit from Thermo Fisher Scientific (Boston, MA) was used to detect and exclude dead cells. All the reagents were tested and titrated for optimum concentration before usage.
Rinaldi S., de Armas L., Dominguez-Rodríguez S., Pallikkuth S., Dinh V., Pan L., Gӓrtner K., Pahwa R., Cotugno N., Rojo P., Nastouli E., Klein N., Foster C., De Rossi A., Giaquinto C., Rossi P., Palma P, & Pahwa S. (2021). T cell immune discriminants of HIV reservoir size in a pediatric cohort of perinatally infected individuals. PLoS Pathogens, 17(4), e1009533.
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5

Multiparametric Flow Cytometric Analysis

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The following fluorochrome conjugated anti-human mAb were used for flow cytometry studies: PD-1 BV421, CD40L BV605, Perforin PE-Dazzle 594 and CD8 PerCP from BioLegend (San Diego, CA); CD3 BUV496, CD4 APC-Cy7, CD69 BV650, IL-2 BV711, CXCR5 Alexa647, IFN-γ PE-Cy7, TNF-α FITC, Granzyme-B and CD27 BV480 from BD Bioscience (San Jose, CA); IL-21 PE from e-Biosciences, (San Diego, CA); and CD45RO-PE-Cy5.5 from Beckman Coulter (Fullerton, CA). Live/Dead® Fixable Blue Dead Cell Stain Kit from ThermoFisher (Boston, MA) was used to detect and exclude dead cells. All the reagents were tested and titrated before usage.
Rinaldi S., Pallikkuth S., Cameron M., de Armas L.R., Cotugno N., Pahwa R., Richardson B., Saini S.R., Rocca S., Lain M.G., Williams S.L., Palma P, & Pahwa S. (2019). Impact of early ART initiation on HIV-specific CD4 and CD8 T cell function in perinatally infected children. Journal of immunology (Baltimore, Md. : 1950), 204(3), 540-549.
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