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Clone id v2lhs 16875 shriok3

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Clone ID V2LHS_16875; shRIOK3 is a laboratory tool designed for genetic manipulation. It functions as a short hairpin RNA (shRNA) expression construct targeting the RIOK3 gene. This product is intended for use in scientific research applications.

Automatically generated - may contain errors

Lab products found in correlation

Puromycin, by Thermo Fisher Scientific (2 mentions) Si02223396, by Qiagen (2 mentions) Siriok3, by Qiagen (2 mentions) Custom sirna oligonucleotides, by Eurogentec (2 mentions) Pgipz plasmids, by Thermo Fisher Scientific (2 mentions) Rhs4743, by Thermo Fisher Scientific (2 mentions) Hiperfect reagent, by Qiagen (2 mentions)

2 protocols using clone id v2lhs 16875 shriok3

1

Silencing RIOK3 and HIF Transcription

Cited 1 time
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Cells were transfected with 20 nM siRNA targeting RIOK3 (SI02223396; siRIOK3) or AllStars Negative control (1027281; siCon) using HiPerFect reagent (Qiagen, Manchester, UK). Custom siRNA oligonucleotides containing 3 sequences targeting HIF-1α (1 CAAGCAACTGTCATATATA, 2 TGCCACCACTGATGAATTA, 3 TGACTCAGCTATTCACCAA) or HIF-2α (1 TAACGACCTGAAGATTGAA, 2 CAAGCCACTGAGCGCAAAT, 3 TGAATTCTACCATGCGCTA) were purchased from Eurogentec (Southampton, UK). pGIPZ plasmids targeting RIOK3 (Clone ID V2LHS_16875; shRIOK3) or non-silencing control shRNAmir (RHS4743; shCon) were obtained from Thermo Scientific. Lentivirus packaging and titration was carried out as described.38 (link) Cells were transduced at MOI 1 and pools of transduced cells were selected with 1 μg/mL puromycin (Life Technologies) 48 h post-transduction.
Singleton D.C., Rouhi P., Zois C.E., Haider S., Li J.L., Kessler B.M., Cao Y, & Harris A.L. (2014). Hypoxic regulation of RIOK3 is a major mechanism for cancer cell invasion and metastasis. Oncogene, 34(36), 4713-4722.
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2

Silencing RIOK3 and HIF Transcription

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were transfected with 20 nM siRNA targeting RIOK3 (SI02223396; siRIOK3) or AllStars Negative control (1027281; siCon) using HiPerFect reagent (Qiagen, Manchester, UK). Custom siRNA oligonucleotides containing 3 sequences targeting HIF-1α (1 CAAGCAACTGTCATATATA, 2 TGCCACCACTGATGAATTA, 3 TGACTCAGCTATTCACCAA) or HIF-2α (1 TAACGACCTGAAGATTGAA, 2 CAAGCCACTGAGCGCAAAT, 3 TGAATTCTACCATGCGCTA) were purchased from Eurogentec (Southampton, UK). pGIPZ plasmids targeting RIOK3 (Clone ID V2LHS_16875; shRIOK3) or non-silencing control shRNAmir (RHS4743; shCon) were obtained from Thermo Scientific. Lentivirus packaging and titration was carried out as described.38 (link) Cells were transduced at MOI 1 and pools of transduced cells were selected with 1 μg/mL puromycin (Life Technologies) 48 h post-transduction.
Singleton D.C., Rouhi P., Zois C.E., Haider S., Li J.L., Kessler B.M., Cao Y, & Harris A.L. (2014). Hypoxic regulation of RIOK3 is a major mechanism for cancer cell invasion and metastasis. Oncogene, 34(36), 4713-4722.
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