N methyl d glucamine

A Trizol kit was purchased from Takara (Tokyo, Japan), and a SYBR Green I Master kit was obtained from Roche (Penzberg, Germany) for reverse transcription‐polymerase chain reaction (RT‐PCR) analyses. Lipofectamine 2000 reagent and Opti‐MEM® were purchased from Invitrogen (Carlsbad, CA, USA) and selected using an RNA interference assay. MACC1 ELISA kit was acquired from CUSABIO (Wuhan, Hubei, China). Reference standard of Epi and NA were from Solarbio (Beijing, China). Aspartic acid, ethylene glycol tetraacetic acid (EGTA), Mg²⁺‐ATP, 5‐nitro‐2‐(3‐phenlypropylamino) benzoic acid, and thapsigarin were acquired from Sigma‐Aldrich (St. Louis, MO, USA) and selected for high performance liquid chromatography coupled with tandem mass spectrometry (HPLC‐MS/MS). Ca(OH)₂, CaCl₂, N‐methyl‐D‐glucamine, and 3,4‐diaminopyridine were purchased from Macklin Biochemical Co. (Shanghai, China) for patch‐clamp recordings. N‐phenylanthtanilic acid and 4‐(2‐hydroxyethyl)‐1‐piperazineethanesulfonic acid (HEPES) were from BBI Life Sciences (Shanghai, China) and used for patch‐clamp recordings. Detailed information of other reagents and antibodies in this work are described in Supplementary Table S1‐2.