Sulfo n hydroxysuccinimide ss biotin

Manufactured by Thermo Fisher Scientific

Sourced in United States

Sulfo-N-hydroxysuccinimide-SS-biotin is a water-soluble biotinylation reagent. It is used for the reversible labeling of proteins and other biomolecules containing primary amine groups.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Protease inhibitor tablet, by Thermo Fisher Scientific (1 mentions) Streptavidin agarose resin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Estradiol 17β glucuronide, by Merck Group (1 mentions) Bicinchoninic acid bca proteinassay kit, by Takara Bio (1 mentions) Estrone 3 sulfate, by Merck Group (1 mentions) Taurocholate, by Merck Group (1 mentions)

Spelling variants of this product

N-hydroxysuccinimide (86 citations) Sulfo-N-hydroxysuccinimide (5 citations) EZ-Link Sulfo- N-hydroxysuccinimide-SS-Biotin (4 citations)

2 protocols using sulfo n hydroxysuccinimide ss biotin

Transporters and Membrane Protein Analysis

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Phenolsulfonphthalein (PSP)
was obtained from Macklin (Shanghai, China). Estradiol-17β-glucuronide
(E17βG), estrone-3-sulfate (E3S), and taurocholate (TCA) were
purchased from Sigma-Aldrich (St. Louis, MO). 4-(4-(Dimethylamino)styryl)-N-methylpyridinium (ASP⁺) and penciclovir (PCV)
were from US Everbright (Suzhou, China) and Aladdin (Shanghai, China),
respectively. Dulbecco’s modified Eagle’s medium (DMEM),
PSP-free DMEM, fetal bovine serum (FBS), trypsin, Lipofectamine 2000,
sulfo-N-hydroxysuccinimide-SS-biotin,
protease inhibitor tablets, and streptavidin-agarose resin were purchased
from Thermo Fisher Scientific (Waltham, MA). Penicillin-streptomycin
was from Hyclone (Logan, UT). The bicinchoninic acid (BCA) protein
assay kit was purchased from Takara (Kyoto, Japan). The first antibodies
for detecting 6-His tag and Na⁺/K⁺-ATPase were
from Proteintech (Rosemont, IL) and Abcam (Boston, MA), respectively.
Their secondary antibodies were from Proteintech (Rosemont, IL).

Wang Z., Li Y., Peng T., Su Y., Luo X., Han W., Zhang H., Ruan J, & Gui C. (2021). Human Organic Anion Transporting Polypeptides 1B1, 1B3, and 2B1 Are Involved in the Hepatic Uptake of Phenolsulfonphthalein. ACS Omega, 6(51), 35844-35851.

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Cell Surface Protein Isolation

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SW480 cells were treated with sulfo-N-hydroxysuccinimide-SS-biotin (1 mg/ml in PBS; Thermo, USA) and lysed with lysis buffer. After centrifugation, the supernatants of lysates were incubated with streptavidin-agarose beads for 1 h at room temperature. After washing, cell surface proteins were recovered from the resin by incubation of the beads with 2 × laemmli buffer containing 100 mM dithiothreitol. Cell membrane proteins were then subjected to SDS-polyacrylamide gel electrophoresis and immunoblot analysis.

Wang M., Zhang Z., Chen M., Lv Y., Tian S., Meng F., Zhang Y., Guo X., Chen Y., Yang M., Li J., Qiu T., Xu F., Li Z., Zhang Q., Yang J., Sun J., Zhang H., Zhang H., Li H, & Wang W. (2023). FDW028, a novel FUT8 inhibitor, impels lysosomal proteolysis of B7-H3 via chaperone-mediated autophagy pathway and exhibits potent efficacy against metastatic colorectal cancer. Cell Death & Disease, 14(8), 495.

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