Zetasizer nano nano s

The polymerization mixture for the preparation of nanoMIPs comprised: NIPAM (39 mg), BIS (2 mg), TBAm (33 mg dissolved in 2 mL of ethanol), AA (2.23 μl for all templates except fumonisin B2) and NAPMA (2.2 mg only for fumonisin B2). The components were dissolved in water (100 mL), sonicated for 5 min, and degassed by bubbling with nitrogen for 30 min. Then 50 mL of this solution was added to 60 g of glass beads bearing the immobilized template. The polymerization was initiated by the addition of a solution (0.5 mL) of APS (60 mg/mL) containing TEMED (30 μL mL⁻¹). The monomer mixture was allowed to polymerize at ambient temperature (20 °C) for 1.5 h. After this time, the beads were transferred into an SPE cartridge (60 ml) fitted with a 20 μm porosity PE frit. Unreacted monomers and other low affinity materials were removed by washing with double-distilled water (10 × 50 mL) at ambient temperature. Next, the temperature was raised to 60 °C and the fractions of high affinity nanoparticles were collected by washing with pre-warmed water at 60 °C (4 × 20 mL). The size of the nanoparticles was determined by dynamic light scattering (DLS) using a Zetasizer Nano (Nano-S) from Malvern Instruments Ltd (Malvern, UK).