Rabbit anti olig2 igg

Manufactured by Merck Group

Sourced in France, Japan

Rabbit anti-Olig2 IgG is an immunoglobulin G (IgG) antibody that is produced in rabbits and specifically targets the Olig2 protein. Olig2 is a transcription factor that plays a crucial role in the development and differentiation of oligodendrocytes, which are cells responsible for forming the myelin sheath around nerve fibers in the central nervous system.

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Lab products found in correlation

Hrp linked anti rabbit igg, by Cell Signaling Technology (1 mentions) Rabbit anti akt igg, by Cell Signaling Technology (1 mentions) Ncl ki67p, by Santa Cruz Biotechnology (1 mentions) Goat anti olig2, by R&D Systems (1 mentions) Rabbit anti ki67 polyclonal antibody, by Leica Biosystems (1 mentions) Mouse anti ecad, by BD (1 mentions) Chick anti gfap, by Merck Group (1 mentions) Mouse anti s100β, by Merck Group (1 mentions) Alexa 488 conjugated anti mouse igg, by Thermo Fisher Scientific (1 mentions) Alexa 568 conjugated anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Biotinylated anti rabbit igg, by Vector Laboratories (1 mentions) Alexa 568 conjugated anti mouse igg, by Thermo Fisher Scientific (1 mentions) Alexa 488 conjugated anti rabbit igg, by Thermo Fisher Scientific (1 mentions)

3 protocols using rabbit anti olig2 igg

Comprehensive Immunostaining Protocol for Neural Cell Types

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Primary antibodies: Rabbit anti-TTR IgG (ThermoFisher, PA580196), 1:200; Rabbit anti-Olig2 IgG (Millipore, AB9610), 1:200; Goat anti-Sox10 IgG (R&D Systems, AF2864), 1:500; Mouse anti-APC/CC1 IgG (Millipore, OP80), 1:50; Rabbit anti-AKT IgG (Cell Signaling Technologies, 4691), 1:1000; Rabbit anti-ERK1/2 IgG (Cell Signaling Technologies, 4695), 1:1000; Rabbit anti-pAKT IgG (Ser473) (Cell Signaling Technologies, 9271), 1:2000; Rabbit anti-pERK1/2 IgG (Thr202/Tyr204) (Cell Signaling Technologies, 4370), 1:2000.
Secondary antibodies: HRP-linked anti-rabbit IgG (Cell Signaling Technologies, 7074), 1:2000–20000; Goat anti-mouse Alexa 488 IgG (ThermoFisher, A10667), 1:500; Goat anti-rabbit Alexa 647 IgG (ThermoFisher (A27040), 1:500; Donkey anti-mouse Alexa 488 IgG (ThermoFisher, A21202), 1:500; Donkey anti-goat Alexa 555 IgG (ThermoFisher, A21432), 1:500; Donkey anti-rabbit Alexa 647 IgG (ThermoFisher, A31573), 1:500.

Alshehri B., Pagnin M., Lee J.Y., Petratos S, & Richardson S.J. (2020). The Role of Transthyretin in Oligodendrocyte Development. Scientific Reports, 10, 4189.

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Comprehensive Antibody Characterization for Cell Lineage Tracing

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Antibodies used in this study are in the followings: mouse anti-E-cad (BD Transduction Laboratories 61081, 1:250); chick anti-GFAP (Millipore, 1:5,000); chick anti-GFP IgY (Abcam ab13970, 1:5,000); rabbit anti-GLAST polyclonal antibody (Covalab (France) PA-INS, 1:1,000); rabbit anti-Ki67 polyclonal antibody (Novocastra, NCL-Ki67p, 1:1,000); goat anti-NeuroD antibody (N-19; Santa Cruz, sc-1,084, 1:1,000); rabbit anti-Olig2 IgG (Millipore, AB9610, 1:1,000); goat anti-Olig2 (R&D systems, 1:1,000); mouse anti-PCNA (Novocastra, PC-10, 1:100); rabbit anti-PSmad3(S423/S425) polyclonal antibody (Millipore 07-1389, 1:1,000); mouse anti-phospho-vimentin IgG (MBL, D076-3S, 1:1,000); goat anti-Prox1 IgG (R&D systems, 1:1,000); mouse anti-S100β (Sigma, 1:1,000); rabbit Sox2 antibody (Millipore, 1:1,000); goat anti-Sox2 IgG (R&D systems, 1:1,000); goat anti-Sox9 polyclonal antibody (R&D systems, 1:1,000).

Ohyama K., Shinohara H.M., Omura S., Kawachi T., Sato T, & Toda K. (2023). PSmad3+/Olig2− expression defines a subpopulation of gfap-GFP+/Sox9+ neural progenitors and radial glia-like cells in mouse dentate gyrus through embryonic and postnatal development. Frontiers in Neuroscience, 17, 1204012.

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Immunohistochemistry and Western Blotting Protocols

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The primary antibodies used in this study were as follows: mouse anti-Nkx2.2 IgG (DSHB), mouse anti-Islet1/2 IgG (DSHB), rabbit anti-Olig2 IgG (Millipore), rabbit anti-Sulf1 IgG (Abcam), mouse anti-Sulf2 IgG (Abcam), and mouse anti-His-tag (MBL, Nagoya, Japan).
For immunohistochemistry, the following secondary antibodies were used: Alexa 488-conjugated anti-rabbit IgG, Alexa 568-conjugated anti-rabbit IgG, Alexa 488-conjugated anti-mouse IgG, Alexa 568-conjugated anti-mouse IgG (Life Technologies), and biotinylated anti-rabbit IgG (Vector Laboratories). For Western blotting, we used anti-mouse IgG-HRP (MP Biomedicals) as the second antibody.

Jiang W., Ishino Y., Hashimoto H., Keino-Masu K., Masu M., Uchimura K., Kadomatsu K., Yoshimura T, & Ikenaka K. (2017). Sulfatase 2 Modulates Fate Change from Motor Neurons to Oligodendrocyte Precursor Cells through Coordinated Regulation of Shh Signaling with Sulfatase 1. Developmental neuroscience, 39(5).

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