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2 protocols using polyoxyethylene sorbitan monostearate tween 60

1

Antimicrobial Activity Evaluation Protocol

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Streptomycin sulfate and phosphate buffer solution (PBS) were purchased from Bio Basic, Canada. Cholesterol, polyoxyethylene sorbitan monostearate (Tween 60), sorbitan monostearate (Span 60), dimethyl sulfoxide (DMSO), and chloroform were bought from Merck, Germany. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, penicillin/streptomycin 100X, Trypsin-EDTA, Trypan blue, RPMI 1640 medium, Dulbecco’s modified Eagle medium (DMEM), fetal bovine serum (FBS) were obtained from Gibco, United States. A dialysis membrane (MWCO 12,000 Da) and MTT (dimethylthiazol-2-yl-)-2,5 were received from Sigma-Aldrich (United States). Mueller Hinton broth, Mueller Hinton agar, barium chloride, and H2SO4 were received from Merck, Germany. Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 15442 were obtained from the Pasteur Institute of Iran.
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2

Formulation and Evaluation of Fusidic Acid Microemulsion

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Fusidic acid micro powder (Joyang laboratories) was gifted by Valor Pharmaceuticals Pvt Limited, Islamabad. Cholesterol was purchased from DAEJUNG Chemicals and Metals co., Korea. Sorbitan monostearate (span 60) and polyoxyethylene sorbitan monostearate (tween 60), chloroform, dihexadecyl phosphate chloride, and Carbopol 934 were purchased from Merck, Germany. Triethanolamine was procured from Sigma Aldrich (St Louis, MO, USA). Distilled water was locally prepared in the Institute of Pharmaceutical Sciences, UVAS, Lahore, Pakistan. All chemicals were of analytical grade.
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