Anti igf 1 antibody

Example 5

Promotion of Expression of Insulin and Insulin-Like Growth Factor

3T3-L1 cells (pre-adipocytes) were seeded at a density of 3×105 cells/well into 6-well plates and grown for 24 hours. Subsequently, the cells were incubated with various concentrations (10 ng-1 μg/ml) of the peptides for 14 days in a 37° C. incubator. Proteins were extracted from cell lysates which were obtained by treatment with cell lysis buffer, quantitatively analyzed, and subjected to Western blotting using an anti-IGF-1 antibody, which is an antibody against the lipolytic marker, and an insulin antibody (Santa Cruz Biotechnology, USA).

From the data, it was observed that the peptide of SEQ ID NO: 7 increased the expression of IGF-1 and insulin in dose-dependent manners (FIG. 23).

Example 5

Promotion of Expression of Insulin and Insulin-like Growth Factor

3T3-L1 cells (pre-adipocytes) were seeded at a density of 3×105 cells/well into 6-well plates and grown for 24 hours. Subsequently, the cells were incubated with various concentrations (10 ng-1 μg/ml) of the peptides for 14 days in a 37° C. incubator. Proteins were extracted from cell lysates which were obtained by treatment with cell lysis buffer, quantitatively analyzed, and subjected to Western blotting using an anti-IGF-1 antibody, which is an antibody against the lipolytic marker, and an insulin antibody (Santa Cruz Biotechnology, USA).

From the data, it was observed that the peptide of SEQ ID NO: 7 increased the expression of IGF-1 and insulin in dose-dependent manners (FIG. 23).

Example 5

Promotion of Expression of Insulin and Insulin-Like Growth Factor

3T3-L1 cells (pre-adipocytes) were seeded at a density of 3×105 cells/well into 6-well plates and grown for 24 hours. Subsequently, the cells were incubated with various concentrations (10 ng-1 μg/ml) of the peptides for 14 days in a 37° C. incubator. Proteins were extracted from cell lysates which were obtained by treatment with cell lysis buffer, quantitatively analyzed, and subjected to Western blotting using an anti-IGF-1 antibody, which is an antibody against the lipolytic marker, and an insulin antibody (Santa Cruz Biotechnology, USA).

From the data, it was observed that the peptide of SEQ ID NO: 7 increased the expression of IGF-1 and insulin in dose-dependent manners (FIG. 23).