Cholesterol (CAS No.: 57-88-5), corn oil, which is the solvent of cholecalciferol (CAS No.: 8001-30-7) and cholecalciferol (CAS No.: 67-97-0) were purchased from Sigma-Aldrich Chemical Company (St. Louis, MO, USA).
Cholecalciferol
Manufactured by Merck Group
Sourced in United States, Germany, France
Cholecalciferol is a form of Vitamin D3 used in the manufacturing of various medical and pharmaceutical products. It serves as a key raw material for the production of vitamin D supplements and other related compounds.
Automatically generated - may contain errors
Lab products found in correlation
Ergosterol, by Merck Group (7 mentions)
Ergocalciferol, by Merck Group (6 mentions)
Acetonitrile, by Thermo Fisher Scientific (5 mentions)
Methanol, by Thermo Fisher Scientific (5 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
Retinyl palmitate, by Merck Group (4 mentions)
Methanol, by Merck Group (4 mentions)
β carotene, by Merck Group (3 mentions)
Ab63856, by Abcam (3 mentions)
Ab14106, by Abcam (3 mentions)
Ab216130, by Abcam (3 mentions)
Ab23981, by Abcam (3 mentions)
Ab25245, by Abcam (3 mentions)
Alexa 488, by Thermo Fisher Scientific (3 mentions)
Dexamethasone (dex), by Merck Group (3 mentions)
Calcium chloride, by Merck Group (3 mentions)
Dextrose, by Merck Group (2 mentions)
Cholesterol, by Merck Group (2 mentions)
Zeaxanthin, by Merck Group (2 mentions)
Lutein, by Merck Group (2 mentions)
53 protocols using cholecalciferol
1
Cholecalciferol Preparation Protocol
2
Cholecalciferol-Induced Vascular Calcification in Asm Knockout Mice
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All animal experiments were approved by local authorities at University of Tuebingen, Charite Berlin and Medical University Vienna (Regierungspräsidium Tuebingen, LAGESO Berlin, BMBWF Vienna). Asm knockout mice (Asm−/−) deficient in Asm activity and corresponding wild-type mice (Asm+/+) were generated by gene targeting described previously [44 (link),45 (link)]. Calcification was induced by high-dosed cholecalciferol treatment [46–48 (link)] in Asm+/+ and Asm−/− mice and in C57BL/6 mice receiving water with control or supplemented with 100 mg/l amitriptyline (Sigma–Aldrich) or 67.5 mg/l fendiline (Santa Cruz Biotechnology) [49 (link)]. Mice were injected subcutaneously with 400000 IU/kg BW of cholecalciferol (Sigma–Aldrich) or vehicle for 3 days [8 (link),37 (link),47 (link)]. After 6 days, blood was obtained by retro-orbital puncture and animals were killed by cervical dislocation during inhalative isoflurane anesthesia and tissues snap-frozen in liquid nitrogen. A photometric method (FUJI FDC 3500i, Sysmex) was employed for determination of serum phosphate and calcium concentrations.
3
Synthesis of Triethylammonium Cholecalciferol Sulfate
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Example 1
6.5 ml of pyridine were added to 1.21 g pyridine-sulphur trioxide complex (ca. 6.76 mmol pyridine complexed with SO3; Sigma-Aldrich; ≥45 wt.-% SO3 according to information by the manufacturer) and 1.21 g (3.14 mmol) cholecalciferol (Sigma-Aldrich) under a nitrogen atmosphere. The solution obtained was intensively stirred for 1 h at 58° C. Then 0.63 ml (0.456 g; 4.55 mmol) of triethylamine were added and stirring was continued for further 20 min. at 58° C.
Then the reaction mixture was cooled in an ice bath at 0° C., 16.5 ml of a cold methanol-trichloromethane (10:1 vol./vol.) solution were added and stirring was continued for 20 min.
The solution was filtered through a glass frit and the solvent was removed on a rotary evaporator. For further purification the residue was twice treated with methanol-trichloromethane (10:1 Vol./Vol.) solution and the solvent was removed on a rotary evaporator, thereby obtaining 1.52 g (3.13 mmol; 99.7%) of triethylammonium cholecalciferol sulfate.
TLC (silica gel): RF=0.42 in methanol-trichloromethan (1:9 vol./vol.).
4
Vitamin D Regulates Breast Cancer Progression
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To assess the impact of VD on breast cancer progression on inflammatory conditions associated with overweight, the mice (n = 8) were fed ad libitum with high-fat diet (245-HF, 45% energy from lipids) (Safe, Augy, France). After 8 weeks of high-fat diet, mice were injected with EO771 cells. Seven days after cell injection, mice received VD (cholecalciferol) (40 IU)/day per mouse; Sigma Aldrich) (Vitamin D group) or vehicle alone (olive oil) (control group) seven times in 2 weeks. This VD concentration (40 IU of VD per mouse) has been reported as being non-toxic to rodents, without major risk of hypercalcemia (38 (link), 41 (link)).
5
Cholecalciferol Formulation Development
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Cholecalciferol (CL, 99% purity), poly (vinyl alcohol) (PVA) (10, 31–50, and 120 kDa), poly (vinyl pyrrolidone) (PVP) (10, 40, and 360 kDa), D‐α‐tocopherol succinate (TS), acetonitrile, and methanol (both of high‐performance liquid chromatography (HPLC) grade) were all purchased from Sigma Aldrich (Poole, Dorset, UK). All other chemicals used were of analytical grade. Millipore HPLC‐grade water was used throughout the study.
6
Cardiovascular Pharmacology Protocol
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The following drugs, salts, and solutions were used: ketamine hydrochloride (Syntec, São Paulo, SP, Brazil), xylazine hydrochloride (Syntec, São Paulo, SP, Brazil), and heparin (Hipolabor, Belo Horizonte, MG, Brazil). Nω-nitro-l -arginine methyl ester hydrochloride (l -NAME), phenylephrine (Phe), sodium nitroprusside, acetylcholine, NaCl, KCl, CaCl2, MgSO4, NaHCO3, KH2PO4, dextrose, ethylenediaminetetraacetic acid, cholesterol, cholecalciferol, colic acid, and methimazole were purchased from Sigma-Aldrich (St. Louis, MO, USA). All of the other reagents were obtained in analytical grade.
7
Carotenoids and Fat-Soluble Vitamin Analysis
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Carotenoids and fat-soluble vitamin standards: E-β-apo-8′-carotenal, α-carotene, 13-Z-β-carotene, cryptoxanthin, lutein, zeaxanthin, astaxanthin, cantaxanthin, β-carotene, 9-Z-β-carotene, retinol, retinol acetate, 25-hydroxycholecalciferol, cholecalciferol and α-tocotrienol were purchased from Sigma-Aldrich (St. Louis, MO, USA). The standard of 5-Z-lycopene was supplied by CaroteNature GmbH (Ostermundigen, Switzerland).
MeOH of LC-MS grade, n-hexane, ethanol and MTBE of HPLC grade, synthetic plasma and butylated hydroxytoluene (BHT) were obtained from Sigma-Aldrich. AMAC and AA of HPLC grade were purchased from Panreac Quimica SA (Barcelona, Spain). Ultrapure water (Milli-Q) was generated by a Millipore system (Millipore, Bedford, MA, USA).
MeOH of LC-MS grade, n-hexane, ethanol and MTBE of HPLC grade, synthetic plasma and butylated hydroxytoluene (BHT) were obtained from Sigma-Aldrich. AMAC and AA of HPLC grade were purchased from Panreac Quimica SA (Barcelona, Spain). Ultrapure water (Milli-Q) was generated by a Millipore system (Millipore, Bedford, MA, USA).
8
Analytical Methods for Vitamins and Nutrients
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Deionized water (18 Mω·cm) from a Millipore Milli-Q (Bedford, MA, USA) water purification system was used to prepare all aqueous solutions. Standard for retinyl esters, cholecalciferol, α-tocopherol acetate, menadione, thiamine, riboflavin, pyridoxine, niacin and pantothenic acid were purchased from Sigma–Aldrich (Fluka, Sigma–Aldrich, Steinheim, Germany). The methanol and acetonitrile of high-performance liquid chromatography (HPLC) grade were used for HPLC analysis and were obtained from Fisher Scientific (Pittsburg, PA, USA). All the other chemicals used were analytical grade and purchased from Sinopharm Chemical Reagent LTD (Beijing, China).
9
Osteogenic Differentiation and Mineralization Assays
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Cholecalciferol (vitamin D3) (C9756, Sigma Aldrich, St. Louis, MO, USA). Mouse monoclonal antibody against α-SMA (ab7817, Abcam, USA), rabbit polyclonal antibodies against acid ceramidase-α (ACα) (sc-292176, Santa cruz, USA), RUNX2 (ab23981, Abcam, USA), OSP (ab63856, Abcam, USA), SM22-α (ab14106, Abcam, USA), VPS16 (Cat. No.17776-1-AP, Protein biotech group, USA), Rab7 (ab137029, Abcam, USA), CD63 (ab216130, Abcam, USA), annexin-II (AnX2, ab41803, USA), and alkaline phosphatase (ALP, sc-28904, Santa Cruz, USA). Rat monoclonal anti-mouse Lamp-1 (ab25245, Abcam, USA), Cre (Cat No. 6905, Novagen EMD Millipore, Billerica MA, USA), and ceramide (MID 15B4, Enzo ALX-804-196-T050). Secondary antibodies are Alexa-488 or Alexa-555-labeled (Life technologies, USA). Von Kossa staining kit (ab150687, Abcam, USA) and Alizarin Red S Solution (TMS-008-C, EMD Millipore. USA) were used for detection AMC.
10
Vitamin D Impacts Breast Cancer
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To assess the impact of VD on breast cancer progression in basal conditions, the mice (n = 8–10) were fed ad libitum with standard diet (AIN-93M, 10% energy from lipids) (Safe, Augy, France) and received by gavage the native form of VD (cholecalciferol) (40 International Units (IU)/day per mouse; Sigma Aldrich) (Vitamin D group) or vehicle alone (olive oil) (control group) seven times in 2 weeks. As a fat-soluble vitamin, VD was diluted in olive oil to ensure better absorption, as previously described (38 (link)–40 (link)). VD supplementation was performed seven days after cell injection.
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