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Fip200

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FIP200 is a protein involved in the formation of autophagosomes, which are double-membrane vesicles that engulf cellular components for degradation. It plays a critical role in the initiation of autophagy, a cellular process that recycles damaged organelles and provides nutrients during periods of starvation.

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Lab products found in correlation

Mouse anti vinculin antibody, by Merck Group (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Spautin 1, by Merck Group (1 mentions) Epirubicin, by Merck Group (1 mentions) Bromodeoxyuridine (brdu), by Merck Group (1 mentions) Bromodeoxyuridine (brdu), by Developmental Studies Hybridoma Bank (1 mentions) Atg13, by Cell Signaling Technology (1 mentions) Goat anti rabbit fitc, by Jackson ImmunoResearch (1 mentions) Cleaved parp, by Cell Signaling Technology (1 mentions) Bafilomycin a1, by Merck Group (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Mitotracker red cmxros, by Thermo Fisher Scientific (1 mentions) Sulphorhodamine b, by Merck Group (1 mentions) Trichloroacetic acid, by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Paclitaxel, by Merck Group (1 mentions) Puromycin, by Merck Group (1 mentions) Cisplatin, by Merck Group (1 mentions) β actin, by Merck Group (1 mentions) Dnase 1, by Roche (1 mentions)

3 protocols using fip200

1

Bone Protein Extraction and Analysis

Cited 2 times
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Protein extracts were prepared from frontal and parietal bones of one-month-old mice after cutting out the sutures. The bone tissues were immersed into liquid nitrogen, grinded to powder, and then lysed using NP40 lysis buffer (PH 8.0 Tris-HCl 20mM, NaCl 137mM, 1% NP40, 10% Glycerol, Na3VO4 1mM). Western blotting was performed and analyzed similar to our previous studies [39 (link)–41 (link)]. The following primary antibodies were used: rabbit anti-RBCC1 (FIP200) antibody (proteintech, 17250-1-AP), rabbit anti-P62 antibody (Enzolife science BML-PW9860-0100), mouse anti-LC3 antibody (Nanotools, NT-0231-100), mouse anti-vinculin antibody (Sigma-aldrich V4505), and rabbit anti-ATG5 antibody (CST, #13007).
Thomas N., Choi H.K., Wei X., Wang L., Mishina Y., Guan J.L, & Liu F. (2019). Autophagy regulates craniofacial bone acquisition. Calcified tissue international, 105(5), 518-530.
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2

Antibody-Based Autophagy and Apoptosis Analysis

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The following antibodies were used: cleaved caspase-3 (Cell Signaling Technology), cleaved PARP (Cell Signaling Technology), LC3B (Cell Signaling Technology), β-actin (Sigma-Aldrich), FIP200 (ProteinTech Group), Atg13 (Cell Signaling Technology), BrdU (Developmental Studies Hybridoma Bank), goat anti-rabbit FITC (Jackson ImmunoResearch Laboratories). The following reagents were used: bafilomycin A1, spautin-1, paclitaxel, epirubicin, cisplatin, puromycin, BrdU, trichloroacetic acid, sulphorhodamine B, protease inhibitor cocktails, DAPI were from Sigma-Aldrich. MitoTracker Green FX (Invitrogen) and MitoTracker Red CMXRos (Invitrogen), Permount SP15-100 Toluene Solution (Fisher Scientific), DNase I (Roche).
Wen J., Yeo S., Wang C., Chen S., Sun S., Haas M.A., Tu W., Jin F, & Guan J.L. (2015). Autophagy inhibition re-sensitizes pulse stimulation-selected paclitaxel-resistant triple negative breast cancer cells to chemotherapy-induced apoptosis. Breast cancer research and treatment, 149(3), 619-629.
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3

Evaluation of Autophagy Markers in Tg-Treated Cells

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Cells were harvested after 30 h of treatment with Tg, unless otherwise stated. Preparation of whole-cell lysates, SDS-PAGE, and immunoblot analysis were performed as described previously [33 (link)]. The following antibodies were used: alpha-tubulin (Abcam, ab7291), ATG5 (Cell Signaling Technology 2630), ATF4 (Cell Signaling Technology 11815), Bip/Grp78 (Cell Signaling Technology 3177), Caspase-3 (8G10) (Cell Signaling Technology 9665), Caspase-8 (1C12) (Cell Signaling Technology 9746), CHOP (Cell Signaling Technology 2895), Cleaved PARP (Asp214) (D64E10) (Cell Signaling Technology 5625), DR5 (Cell Signaling Technology 8074), FADD (G-4) (Santa Cruz sc-271748), FIP200 (Proteintech 17250–1-AP, GABARAP (Proteintech PM037), GABARAPL1 (Abcam ab86497), GABARAPL2 (Proteintech PM038), p-JNK (Cell Signaling Technology 9251), JNK (Cell Signaling Technology 9252), LC3B (Cell Signaling Technology 2775), PERK (Cell Signaling Technology 5683), XBP1s (BioLegend 647502).
Lindner P., Christensen S.B., Nissen P., Møller J.V, & Engedal N. (2020). Cell death induced by the ER stressor thapsigargin involves death receptor 5, a non-autophagic function of MAP1LC3B, and distinct contributions from unfolded protein response components. Cell Communication and Signaling : CCS, 18, 12.
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