Iba 1 antibody

The efficacy of RDN in individual rats was evaluated for tyrosine hydroxylase (TH) expression in the renal vessel and renal cortex tissue sections by immunohistochemistry using anti-TH antibody (Servicebio, Wuhan, China). The intensity of anti-TH staining was determined by measuring their integrated optical density (IOD). The contents of microglia in the cerebral cortex tissues were determined by immunohistochemistry using anti-ionized calcium binding adaptor molecule-1 (Iba-1) antibody (Servicebio).

An anti-ionized calcium binding adaptor molecule-1 (Iba-1) antibody (Servicebio, Wuhan, China) was used to specifically recognize microglia in the cerebral cortex. All sections were examined using a microscope (Nikon, Tokyo, Japan), and at least five random fields (×400) per section were analyzed.

The hippocampus was carefully removed and fixed immediately in 10% neutral buffered formalin, then embedded with paraffin. The paraffin-embedded hippocampus was cut into 4 µm sections for immunofluorescence staining as previously described [30 (link)]. The Iba-1 antibody (Servicebio, Wuhan, China) was stained to detect microglial cells, followed by secondary antibodies of CY-3-conjugated goat anti-rabbit IgG (Servicebio, Wuhan, China). Moreover, the DAPI (Servicebio, Wuhan, China) was used for nuclear staining. The Image-Pro Plus 3.0 software (Media Cybernetics, Silver Spring, MD, USA) was used to mark cells in 6 randomly selected 200× fields [31 (link),32 (link)] and the positive cells were quantified manually [33 (link)].