Countess 2 fl reusable slides

To measure the change in cell viability after the treatment, cells were washed with PBS and detached by trypsinization. Cells were kept in a 37°C incubator for trypsinization for 5 mins and were resuspended in fresh medium. The cell pellets were collected after centrifuging them at 400 g for 5 mins at 4°C, the supernatants were discarded, and cold PBS was added to resuspend the cells. Cells were then aliquoted in small volumes and trypan blue was used to stain in a 1:1 ratio to count the number of viable cells in each sample using Countess™ II FL reusable slides and the countess™ II cell counter (Thermo Fisher, Waltham, MA, USA). In total, 4 readings were taken for each sample and the averages (cells/mL) were multiplied by the respective volume of each cell suspension to get the total number of living cells per treated sample. The total number of living cells after FIR treatment were normalized to IR- sample (21 (link)).