1 3 dimethylaminopropyl 3 ethylcarbodiimide hydrochloride edc hcl

Manufactured by Merck Group

Sourced in United States

1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl) is a water-soluble carbodiimide compound commonly used as a coupling agent in biochemistry and organic synthesis. It facilitates the formation of amide bonds between carboxyl groups and primary amines.

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6 protocols using 1 3 dimethylaminopropyl 3 ethylcarbodiimide hydrochloride edc hcl

Biomolecule Conjugation Using EDC/sulfoNHS

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Citric acid, ethylenediamine, and 1-(3-(dimethylamino)propyl)-3 ethylcarbodiimide hydrochloride (EDC·HCl) were purchased from Merck and the purity is almost 99.5%. N-Hydroxysulfosuccinimide sodium salt (sulfoNHS) (98%) and hemin (96%) were purchased from Sigma-Aldrich. It is clear from the database of Sigma-Aldrich that hemin contains Fe(iii) ions. Other chemicals were purchased from the following companies: BSA from MP Biomedicals which contains fatty acids <0.05 mg g⁻¹, 2,2,2-trifluoroethanol (TFE) from Apollo Scientific (98%), and β-mercaptoethanol from Alfa Aesar (98%).

Mondal S., Ghorai N., Bhunia S., Ghosh H.N, & Amdursky N. (2021). Long-range light-modulated charge transport across the molecular heterostructure doped protein biopolymers. Chemical Science, 12(25), 8731-8739.

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Cadmium-based Nanostructure Synthesis

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Reagents and chemicals H3PO4 (85%), H2SO4 (95 -97%), graphite (99.9%), KMnO4, ethanol (99.6%), H2O2 (30%), HNO3 (65%), iron(III) chloride hexahydrate (FeCl3•6H2O), FeCl2•4H2O, NH3 (25%), N,Ndimethylacetamide, ED (ethane-1,2-diamine), NHS (N-hydroxysuccinimide), ethylenediamine, 1-(3-dimethyl amino propyl)-3-ethylcarbodiimide hydrochloride (EDC•HCl) and cadmium chloride (CdCl2) were purchased from Merck (Darmstadt, Germany), Merck (Darmstadt, Germany). Cadmium chloride was used for the preparation of a stock solution of Cd 2+ .

Mehdinia A., Salamat M, & Jabbari A. (2020). Amino-modified Graphene Oxide/Fe₃O₄ for Dispersive Solid-Phase Extraction of Cadmium Ions in Rice, Lentil, and Water Samples. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 36(3).

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Fluorescent Labeling of Proteins Using QDs

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Orange–red (λ_em = 610 nm, particle size = 24 nm) ZnCdSe/ZnS QDs were purchased from Wuhan Jiayuan Quantum Dots Corporation, Ltd. (Wuhan, China). 1-(3-dimethylaminopropyl)-3-ethylcar-bodiimide hydrochloride (EDC-HCl) and Bovine serum albumin (BSA) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). BCA Protein Assay Kits (PC0020) were purchased from Sorlarbio Life Sciences (Beijing, China). Staphylococcal protein A (SPA) was purchased from Beijing Bersee Science and Technology Corporation, Ltd. (Beijing, China). HRP-conjugated anti-His-Tag monoclonal antibody (5C3) and HRP-conjugated goat anti-mouse IgG were purchased from Abbkine Scientific Corporation, Ltd. (Wuhan, China). eECL Western Blot Kit was purchased from CWbiotech Corporation, Ltd. (Jiangsu, China). Nitrocellulose membranes, glass fiber and absorbent pads were purchased from Millipore (Bedford, MA, USA).

Li Z., Wang A., Zhou J., Chen Y., Liu H., Liu Y., Zhang Y., Ding P., Zhu X., Liang C., Qi Y., Liu E, & Zhang G. (2022). A Universal Fluorescent Immunochromatography Assay Based on Quantum Dot Nanoparticles for the Rapid Detection of Specific Antibodies against SARS-CoV-2 Nucleocapsid Protein. International Journal of Molecular Sciences, 23(11), 6225.

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PEG-based Drug Delivery System Synthesis

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Polyethylene glycol (PEG, average molecular weight ∼38 kDa), allyl glycidyl ether (AGE), mercaptoethanol, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl) and N-hydroxysuccinimide (NHS) were purchased from Sigma-Aldrich (USA). Sodium hydride (NaH) was supplied by Damao Chemical Reagent Factory (Tianjin, China). 5-Fluorouracil (USP29) was purchased from Nantong Jinghua Pharmaceuticals Co., Ltd (Jiangsu, China). All other chemicals used were of reagent grade.

Li M., Liang Z., Sun X., Gong T, & Zhang Z. (2014). A Polymeric Prodrug of 5-Fluorouracil-1-Acetic Acid Using a Multi-Hydroxyl Polyethylene Glycol Derivative as the Drug Carrier. PLoS ONE, 9(11), e112888.

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Fabrication and Characterization of PLGA-Chitosan Biomaterials

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Poly(d,l-lactide-co-glycolide) (PLGA, M_W ≈ 200,000) with a –COOH end-group was purchased from the (Shandong Institute of Medical Instruments, Jinan, China). Chitosan (M_W ≈ 200 kDa, with a deacetylation degree (DD) of 95%) was purchased from the Biochemical Medicine Plant of Qingdao (Qingdao, China). N-hydroxysuccinimide (NHS), hexafluoroisopropanol (HFIP), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl) and 2-morpholinoethane sulfonic acid (MES) were purchased from Sigma Aldrich (St. Louis, MO, USA). Staphylococcus aureus (ATCC 25923) and Pseudomonas aeruginosa (ATCC 9027) were maintained in our laboratory. Human dermal fibroblast cells (HDFs) and keratinocyte cells (HaCaTs) were purchased from the (Kunming Animal Institute, Kunming, China).

Yang S., Han X., Jia Y., Zhang H, & Tang T. (2017). Hydroxypropyltrimethyl Ammonium Chloride Chitosan Functionalized-PLGA Electrospun Fibrous Membranes as Antibacterial Wound Dressing: In Vitro and In Vivo Evaluation. Polymers, 9(12), 697.

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Synthesis and Characterization of Photodynamic Nanoparticles

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2,6-diaminopyridine, FeCl₃·6H₂O and Sodium hyaluronate were commercially provided by Shanghai Aladdin Reagent CO, Ltd. (China). N-hydroxysuccinimide (NHS) and 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl) were brought from Sigma-Aldrich Trading Co., Ltd. (China). [Ru(dpp)₃]Cl₂ (RDPP), 1,3-diphenylisobenzofuran (DPBF) and 2′,7′-dichlorofluorescein diacetate (DCFH-DA)were purchased from Shanghai Medpep Co., Ltd (China). Roswell Park Memorial Institute (RPMI) 1640, fetal bovine serum (FBS), penicillin–streptomycin, and trypsin were supplied by GIBCO Invitrogen Corp. (USA). Cell counting kit-8 (CCK-8) and 4′,6-Diamidino-2-phenylindole (DAPI) were provided by Shanghai Yeasen Biotech Co., Ltd. (China). All other reagents were received and used without further purification. Deionized (DI) water was obtained from experimental water purification system.

Shu F., Yang T., Zhang X., Chen W., Wu K., Luo J., Zhou X., Liu G., Lu J, & Mao X. (2021). Hyaluronic acid modified covalent organic polymers for efficient targeted and oxygen-evolved phototherapy. Journal of Nanobiotechnology, 19, 4.

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