The antibody against γ-protocadherin (γ-Pcdh) was from Synaptic Systems (Gottingen, Germany). The antibody against VDAC was from Cell Signaling (Danvers, MA, United States). Antibodies against GAPDH, PSD95, Rab11, and TrkB were from Millipore (Billerica, MA, United States). Antibodies against Slit2 and γ-adaptin were was from Proteintech (Rosemont, IL, United States). Antibodies against MEA6 and calbindin were from Sigma (St. Louis, MO, United States). Antibodies against Bip, PDI, Robo2, and Semaphorin 3A were from Abcam (Cambridge, United Kingdom). Antibodies against β-actin, β-tubulin, YY1, and BDNF were from Santa Cruz (Dallas, TX, United States). Goat anti-mouse and anti-rabbit IgG horseradish peroxidase-conjugated were from Thermo Fisher Scientific (Waltham, MA, United States). The alexa fluor-conjugated secondary antibody was from Invitrogen (Carlsbad, CA, United States). Protease inhibitor cocktail was from Roche (Mannheim, Germany). Other chemicals were from Sigma unless stated otherwise.
Horseradish peroxidase conjugated goat anti rabbit and anti mouse igg
Manufactured by Thermo Fisher Scientific
Sourced in United States
Horseradish peroxidase-conjugated goat anti-rabbit and anti-mouse IgG is a reagent used in various immunoassay and immunoblotting techniques. The product contains a secondary antibody that binds to rabbit or mouse primary antibodies and is conjugated with the enzyme horseradish peroxidase, which can be detected through a colorimetric or chemiluminescent reaction.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions)
Protease inhibitor cocktail, by Roche (2 mentions)
Mouse monoclonal anti β actin, by Abcam (2 mentions)
Fk866, by Adipogen (2 mentions)
Mouse monoclonal anti trf1, by Santa Cruz Biotechnology (2 mentions)
Rabbit anti phospho ser139 histone h2ax γh2ax, by Cell Signaling Technology (2 mentions)
Mouse monoclonal anti nf κb p65, by Santa Cruz Biotechnology (2 mentions)
Rabbit anti p21, by Santa Cruz Biotechnology (2 mentions)
Alexa fluor 488 conjugated goat anti mouse igg, by Thermo Fisher Scientific (2 mentions)
Alexa fluor 594 conjugated goat anti rabbit igg, by Thermo Fisher Scientific (2 mentions)
Brain derived neurotrophic factor (bdnf), by Santa Cruz Biotechnology (1 mentions)
Antibodies against gapdh, by Merck Group (1 mentions)
Nissl, by Beyotime (1 mentions)
5 bromo 2 deoxyuridine brdu, by Abcam (1 mentions)
Neuronal nuclei (neun), by Merck Group (1 mentions)
Synapsin 1, by Abcam (1 mentions)
Glua1, by Merck Group (1 mentions)
Glua2, by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Munc13 1, by Synaptic Systems (1 mentions)
5 protocols using horseradish peroxidase conjugated goat anti rabbit and anti mouse igg
1
Antibody Sourcing for Protein Analysis
2
Comprehensive Neuronal Marker Antibody Panel
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Antibodies against GAPDH, GluA1, GluA2, NeuN, and synaptophysin were from Millipore (Billerica, MA, United States). Antibodies against Bip, Robo2, Sema6A, Synapsin-1, Munc18-1, and 5-bromo-2′-deoxyuridine (BrdU) were from Abcam (Cambridge, United Kingdom). Antibodies against γ-protocadherin (γ-pcdh), Rab3A, Rim1, and Munc13-1 were from Synaptic Systems (Gottingen, Germany). Antibody against Slit2 was from Proteintech (Rosemont, IL, United States). Antibody against TrkB was from Cell Signaling (Danvers, MA, United States). Anti-vesicular glutamate transporter 1 (vGluT1) antibody was a gift from Dr. Masahiko Watanabe (Hokkaido University, Sapporo, Japan). Antibodies against both Mea6 and calbindin were from Sigma-Aldrich (St. Louis, MO, United States). Antibodies to β-tubulin and brain-derived neurotrophic factor (BDNF) were from Santa Cruz Biotechnology (Dallas, TX, United States). Goat anti-mouse and anti-rabbit IgG horseradish peroxidase-conjugated were from Thermo Fisher (Waltham, MA, United States). DAPI and Alexa Fluor-conjugated secondary antibody was from Invitrogen (Carlsbad, CA, United States). Protease inhibitor cocktail was from Roche (Mannheim, Germany). Nissl was from Beyotime (Shanghai, China). Other chemicals were from Sigma unless stated otherwise.
3
Investigating Signaling Pathways in Cancer
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GRP and RC-3095 were purchased from Sigma. AG490 and WP1066 were supplied by Calbiochem and Sigma, respectively. The antibodies for GRP-R and MMP-9, were supplied by Santa Cruz Biotechnology. The antibodies for phospho-JAK2, JAK2, phospho-STAT3, and STAT3 were obtained from Cell Signaling. MMP-2 and β-actin antibodies were purchased from Abcam and Bioworld Technology, respectively. Horseradish peroxidase-conjugated goat anti-rabbit and anti-mouse IgG were obtained from Thermo Fisher Scientific.
4
Analyzing Cell Signaling Markers
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Hydrogen peroxide (H2O2) was obtained from Junsei (Tokyo, Japan). FK866 was purchased from Adipogen (Switzerland). The antibodies used in the present study were as follows—rabbit anti-p21 (Santa Cruz Biotech, Dallas, TX, USA), mouse monoclonal anti-p53 (Calbiochem, San Diego, CA, USA), mouse monoclonal anti-β-Actin (Abcam, Cambridge, MA, USA), mouse monoclonal anti-TRF1 (Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-phospho (Ser139)-histone H2AX (γH2AX) (Cell Signaling Technology, Danvers, MA, USA), mouse monoclonal anti-NF-κB p65 (Santa Cruz Biotech, Dallas, TX, USA), horseradish peroxidase-conjugated goat anti-rabbit, and anti-mouse IgG (Thermo Fisher Scientific, Waltham, MA, USA), Alexa Fluor® 488-conjugated goat anti-mouse IgG, and Alexa Fluor® 594-conjugated goat anti-rabbit IgG (Invitrogen, Camarillo, CA, USA).
5
Visfatin Regulation and Oxidative Stress
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H2O2 was obtained from Junsei (Tokyo, Japan). Visfatin and FK866 were purchased from Adipogen (San Diego, CA, USA). Primary antibodies used in this study were as follows: rabbit anti-Visfatin (Adipogen, San Diego, CA, USA), rabbit anti-p21 (Santa Cruz Biotech, Dallas, TX, USA), mouse monoclonal anti-p53 (Calbiochem, San Diego, CA, USA), rabbit anti-α-Tubulin (Bioworld, Minneapolis, MN, USA), mouse monoclonal anti-β-Actin (Abcam, Cambridge, MA, USA), mouse monoclonal anti-c-Myc (Santa Cruz Biotech, Dallas, TX, USA), mouse monoclonal anti-TRF-1 (Santa Cruz Biotech, Dallas, TX, USA), rabbit anti-phospho (Ser139)-histone H2AX (γH2AX) (Cell Signaling Technology, Danvers, MA, USA), and mouse monoclonal anti-NF-κB p65 (Santa Cruz Biotech, Dallas, TX, USA). Horseradish peroxidase-conjugated goat anti-rabbit and anti-mouse IgG were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Alexa Fluor® 488-conjugated goat anti-mouse IgG, Alexa Fluor® 488-conjugated goat anti-rabbit IgG, Alexa Fluor® 594-conjugated goat anti-mouse IgG, and Alexa Fluor® 594-conjugated goat anti-rabbit IgG were purchased from Invitrogen (Camarillo, CA, USA).
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