Goat anti mouse igg hrp conjugate

Staining with anti‐CD20, CD10, BCL‐6, and MUM‐1 antibodies was performed as described previously.³⁶ Briefly, mouse anti‐CD20 (L26; dilution 1:200; Invitrogen), mouse anti‐CD10 (56C6; dilution 1:1; Invitrogen), mouse anti‐BCL‐6 (D‐8; dilution 1:75; Santa Cruz), and mouse anti‐MUM‐1 (MUM1p; dilution 1:10; Dako) antibodies were used as the primary antibody in a 1‐hour reaction at room temperature and a goat anti‐mouse IgG HRP conjugate (dilution 1:500; ProteinTech) was then used as the secondary antibody.

Polyethylenimine (PEI) (764582, Sigma-Aldrich, St. Louis, MO, USA) and jetPRIME (114-15, Polyplus, Strasbourg, France) were used for transfection. DSS (Cat# D8906) was purchased from Sigma-Aldrich. Listeria monocytogenes were provided by the laboratory of Zengfan Jiang, Peking University. The antibodies used in this study were as follows: Anti-OTUD6A (Proteintech, Rosemont, IL, USA, Cat# 24486-1-AP); Anti-NF-κB P65 (CST, Danvers, MA, USA, Cat# 8242); Anti-p-p65 (Ser536) (93H1) (CST, Cat# 3033); Anti-IκBα (CST, Cat# 9242); Anti-p-IκBα (CST, Cat# 5209); Goat anti-mouse IgG, HRP conjugate (Proteintech, Cat# SA00001-1); and Goat anti-rabbit IgG, HRP conjugate (Proteintech, Cat# SA00001-2).