Branched PEI (Mw ~25 kDa, average Mn ~10 kDa) and poly(D,L-lactic acid) (Mw ~15 kDa) were obtained from Sigma-Aldrich and Polysciences Inc. (Germany), respectively. Acryloyl chloride was purchased from TCI (Shanghai) Development Co., Ltd. (Shanghai, China). Triethylamine was provided by Sigma-Aldrich. siRNA targeting PKM2 (siPKM2): 5′-CCAUAAUCGUCCUCACCAA-3′ (sense), and negative control siRNA (siNC) were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China). Cy5-labled siRNA targeting PKM2 was provided by GenePharma Co. (Shanghai, China). Bicinchoninic acid (BCA) assay kit was obtained from Beyotime Institute of Biotechnology (Shanghai, China). Lipofectamine® 2000 (Lipo 2000) Reagent was provided by Invitrogen Life Technologies (Carlsbad, CA, USA). Primary antibodies against PKM2 was purchased from Cell Signaling Technology (Danvers, MA, USA). All other organic solvents used were of analytical grade. HCT116, HepG2 and SKOV3 cells were obtained from Shanghai Cell Bank of Chinese Academy of Sciences (Shanghai, China).
Lipofectamine 2000 lipo 2000 reagent
Manufactured by Thermo Fisher Scientific
Sourced in United States
Lipofectamine 2000 (Lipo 2000) is a cationic lipid-based transfection reagent for the introduction of nucleic acids into eukaryotic cells. It facilitates the delivery of DNA, RNA, and other macromolecules into cells for various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Polyvinylidene difluoride pvdf membrane, by Merck Group (3 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (2 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Enhanced chemiluminescence reagent, by Thermo Fisher Scientific (2 mentions)
Horseradish peroxidase conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions)
Branched pei, by Merck Group (1 mentions)
Bicinchoninic acid bca assay kit, by Beyotime (1 mentions)
Triethylamine, by Merck Group (1 mentions)
Hct116, by Shanghai Cell Bank (1 mentions)
Hepg2, by Shanghai Cell Bank (1 mentions)
Skov3, by Shanghai Cell Bank (1 mentions)
Anti pparγ, by Abcam (1 mentions)
Pgc 1α, by Novus Biologicals (1 mentions)
Anti cd63, by Abcam (1 mentions)
Gapdh, by Proteintech (1 mentions)
Cald1, by Proteintech (1 mentions)
Prestained protein marker, by Thermo Fisher Scientific (1 mentions)
Mir 124 inhibitor, by RiboBio (1 mentions)
5 protocols using lipofectamine 2000 lipo 2000 reagent
1
Branched PEI and PDLLA Nanocarrier for siRNA Delivery
2
CD151-mediated Lipid Metabolism Regulation
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Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were purchased from GIBCO (Grand Island, NY). Lipofectamine 2000 (Lipo 2000) reagent was obtained from Invitrogen (Carlsbad, CA). CD151 small interfering RNA (siCD151) and scrambled siRNA controls were synthesized by Vigenebio (Shandong, China). Real-time PCR primers of mRNA were synthesized and purchased from Wuhan AuGCT DNA-SYN Biotechnology Co., Ltd. (Wuhan, China). Antibodies against CD151 (Cat No: 66567-1-Ig), CD9 (Cat No: 60232-1-Ig) and GAPDH (Cat No: 60004-1-Ig) were purchased from Proteintech (Chicago, IL). Anti-PPAR-α (Cat No: NB300-537), and peroxisome proliferator-activated receptor coactivator 1α (PGC-1α) (Cat No: NB300-537) were purchased from Novus (Brazil, SP). Anti-CD151 (Cat No: ab33315), Anti-CD63(Cat No: ab134045), Anti-PPAR-γ (Cat No: ab59256) were obtained from Abcam (Cambridge, MA). Recombinant CD151 adenovirus (Adeno-CD151;1.02 × 1011 plaque-forming-unit/mL), empty adenovirus (Adeno-CMV-3*flag-tagged) were generated by Shanghai Obio Technology Co. Ltd. (Shanghai, China). FITC-dextran (Cat No: 46945) and other reagents were purchased from the Sigma-Aldrich Company, unless otherwise specified.
3
Investigating miR-103-3p Regulation in Cardiac Biology
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Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY). Lipofectamine 2000 (Lipo2000) reagent was obtained from Invitrogen (Carlsbad, CA). miR-103-3p mimics, miR-103-3p inhibitor, biotin-labeled miR-103-3p, and antisense oligonucleotide (ASO) for lnccytb were purchased from RiboBio (Guangzhou, China). The primers of miR-103-3p and U6 were also acquired from RiboBio (Guangzhou, China). Real-time PCR primers for mRNA were synthesized by Augct (Wuhan, China). Antibodies against GFP (cat. no. AE012), VDAC1 (cat. no. A15735), and PTEN (cat. no. A19104) were purchased from ABclonal (Wuhan, China). Anti-GAPDH (cat. no. 60004-1-Ig) and anti-ANP (cat no. 27426-1-AP) were purchased from Proteintech (Chicago, IL). Phospho-Akt (Ser473) (cat. no. 4060) and Akt (cat. no. 4691) were purchased from Cell Signaling Technology (Beverly, MA). Polyvinylidene difluoride (PVDF) membranes were purchased from Millipore (Darmstadt, Germany). FITC-phalloidin (cat. no. P5282) and other chemical reagents were purchased from Sigma-Aldrich (Shanghai, China). Adeno-associated virus (AAV) and adeno virus were purchased from WZ Biosciences (Shandong, China).
4
Molecular Pathway Profiling in Cells
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RPMI-1640, Dulbecco’s Modified Eagle Medium (DMEM), and fetal bovine serum (FBS) were obtained from GIBCO (Grand Island, NY). Lipofectamine 2000 (Lipo2000) reagent was obtained from Invitrogen (Life Technologies Corporation, Carlsbad, CA). The primers for miR-320a and U6 real-time PCR, miR-320a mimics, miR-320a inhibitor, and their controls were purchased from RiboBio (Guangzhou, China). Antibodies against MafF (Cat No: 12771-1-AP) and CALD1 (Cat No: 66693-1-lg) were procured from Proteintech (Chicago, MA). Antibodies against DDRGK1 (Cat No: bs-8252R) and KLHL36 (Cat No: bs-9628R) were purchased from Bioss (Beijing, China). Antibodies against AKT (Cat No: 4691T) and p-AKT (Cat No: 13038T) were purchased from CST (Cell Signaling Technology, Shanghai, China). Polyvinylidene difluoride (PVDF) membranes were purchased from Millipore (Merck KGaA, Darmstadt, Germany). Horseradish peroxidase-conjugated secondary antibodies and enhanced chemiluminescence reagents were obtained from Pierce Biotechnology (Thermo Fisher Scientific Inc., Rockford, IL). Other reagents were purchased from the Sigma-Aldrich Company, unless otherwise specified.
5
Evaluating miR-124 and CD151 Expression
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RPMI-1640, Dulbecco's Modified Eagle Medium (DMEM), and fetal bovine serum (FBS) were obtained from GIBCO (Grand Island, NY). Lipofectamine 2000 (Lipo 2000) reagent was obtained from Invitrogen (Life Technologies Corporation, Carlsbad, CA). The primers for miR-124 and U6 real-time PCR, miR-124 mimics, miR-124 inhibitor, CD151 siRNA, and their controls were purchased from RiboBio (Guangzhou, China). Antibody against CD151 (Cat No: ab33315) was procured from Abcam (Cambridge, MA). Antibody against Phospho-NOS3-S1177 (Cat No: AP0421) was purchased from ABclonal Biotech (Cambridge, MA). Prestained protein markers were obtained from Fermentas (Thermo Fisher Scientific Inc., Rockford, IL). Polyvinylidene difluoride (PVDF) membranes were from Millipore (Merck KGaA, Darmstadt, Germany). Horseradish peroxidase-conjugated secondary antibodies and enhanced chemiluminescence reagents were from Pierce Biotechnology (Thermo Fisher Scientific Inc., Rockford, IL). Alexa Fluor® 594 Donkey Anti-Mouse IgG (H+L) Antibody (Cat No: A-21203) were obtained from MOLECULAR PROBES (Thermo Fisher Scientific Inc., Rockford, IL). Other reagents were purchased from the Sigma-Aldrich Company, unless otherwise specified.
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