levels of VEGF, TNF-α, and IL-6 were measured by the
enzyme-linked immunosorbent assay (ELISA) method. They were measured
with ELISA Kits Rat VEGF (RRV00; R&D; USA; Inc), Rat TNF-α
(Dy510; R&D; USA; Inc), and Rat IL-6 (R6000B; R7D; USA; Inc).
Rat VEGF (RRV00) is a recombinant protein produced in E. coli. It is a member of the VEGF family and functions as a potent mitogen for endothelial cells.
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