Polymyxin b sulphate pol b

Polymyxin B sulphate (Pol-B) was purchased from Alfa Aesar®. Sodium poly(styrene sulphonate) 70 KDa average molecular weight (PSS), 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES), phosphotungstic acid hydrate and Luria Bertani (LB) broth (Miller) were bought from Sigma-Aldrich Co. Nylon 0.45 μm syringe filters were purchased from Camlab Ltd. Carbon-coated copper TEM grids (200 mesh) were purchased from Agar Scientific Ltd. Float-a-Lyzer® G2 (20 KDa MWCO) dialysis membranes were purchased from Spectrum Laboratories Inc. Dulbecco’s phosphate buffered saline (DPBS), 9.5 mM in phosphate and without Ca²⁺ and Mg²⁺, was bought from Lonza. Agar was purchased from Sigma-Aldrich Co.

Polymyxin B sulphate (Pol-B) was purchased from Alfa Aesar®. Sodium poly(styrene sulphonate) (PSS) of 32, 17 and 10 kDa average Mw (GPC standards), 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES), phosphotungstic acid hydrate, agar and Luria Bertani (LB) broth (Miller) were bought from Sigma-Aldrich Ltd. Nylon 0.45 µm syringe filters were purchased from Camlab Ltd. Carbon-coated nickel TEM grids (200 mesh) were purchased from Agar Scientific Ltd. Dulbecco’s phosphate buffered saline (DPBS), 9.5 mM in phosphate and without Ca²⁺ and Mg²⁺, was bought from Lonza.