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K3edta tubes

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K3EDTA tubes are blood collection tubes used for the stabilization and preservation of blood samples. The tubes contain tripotassium ethylenediaminetetraacetic acid (K3EDTA) as an anticoagulant, which helps prevent blood from clotting during the collection and transportation process. These tubes are designed to ensure the integrity of the collected blood sample for downstream laboratory analysis.

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Lab products found in correlation

Magnetic luminex performance multiplex assay, by R&D Systems (2 mentions) 7 plex human high sensitivity cytokine premixed kit, by R&D Systems (2 mentions)

3 protocols using k3edta tubes

1

Cardiac Puncture Blood Preparation

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Blood was collected via cardiac puncture and placed into K3-EDTA tubes (Thermo Fisher Scientific) and mixed well to avoid clotting. Blood was then resuspended in 10 ml RBC lysis buffer and placed on a rocker for 5 min. Following termination of RBC lysis in 40 ml PBS, the suspension was then spun down and resuspended in PBS.
Mardilovich K., Naylor G., Julian L., Phinichkusolchit N., Keeshan K., Blyth K, & Olson M.F. (2024). Caspase-resistant ROCK1 expression prolongs survival of Eµ-Myc B cell lymphoma mice. Disease Models & Mechanisms, 17(5), dmm050631.
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2

Multiplex Cytokine Profiling in Plasma

Cited 1 time
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Blood samples were collected in 2ml K3EDTA tubes (ThermoFisher Scientific, Massachusetts, USA). On arrival to the laboratory, samples were centrifuged at 1500g for 15 minutes at room temperature, and plasma removed and frozen at -80°C until processing for cytokine measurement. All candidate proteins were measured utilizing Magnetic Luminex® Performance Multiplex Assay (R&D Systems Inc., Minneapolis, USA) using the customised 7-plex Human High Sensitivity Cytokine Premixed kit (R&D, FCSTM14). From the cytokines available for “high-sensitivity” measurement, we chose five cytokines known to be stimulated by IFN-α (interleukin-1 beta (IL-1β), interleukin-2 (IL-2), interleukin-6 (IL-6), interferon-gamma (IFN-γ), and tumor necrosis factor-alpha (TNF-α) (Taylor and Grossberg, 1998 (link))) and two that are inhibited (interleukin-7 (IL-7) (Su et al, 1997 (link)) and interleukin-17A (IL-17A) (Cui et al, 2014 (link))). All samples were assayed according to the manufacturer’s protocol, and the results analyzed using SoftMax Pro V4.8 (full details in Supplemental Materials). IFN-γ levels were below detection limit for most samples, therefore were not analysed further.
Hepgul N., Cattaneo A., Agarwal K., Baraldi S., Borsini A., Bufalino C., Forton D.M., Mondelli V., Nikkheslat N., Lopizzo N., Riva M.A., Russell A., Hotopf M, & Pariante C.M. (2016). Transcriptomics in Interferon-α-Treated Patients Identifies Inflammation-, Neuroplasticity- and Oxidative Stress-Related Signatures as Predictors and Correlates of Depression. Neuropsychopharmacology, 41(10), 2502-2511.
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3

Quantifying Cytokine Profiles in Plasma Samples

Cited 1 time
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Blood samples were collected in 2 ml K3EDTA tubes (ThermoFisher Scientific). On arrival to the laboratory, samples were centrifuged at 1500 g for 15 min at room temperature and plasma were removed and frozen at −80 °C until processing for cytokine measurement. All candidate proteins were measured using Magnetic Luminex Performance Multiplex Assay (R&D Systems, Minneapolis, USA), using the customized 7-plex Human High Sensitivity Cytokine Premixed kit (R&D, FCSTM14). From the cytokines available for ‘high-sensitivity' measurement, we chose five cytokines known to be stimulated by IFN-α (IL-1β, IL-2, IL-6, IFN-γ, and TNF-α (Taylor and Grossberg, 1998 )) and two that are inhibited (IL-7 (Su et al, 1997 (link)) and IL-17A (Cui et al, 2014 (link))). All samples were assayed according to the manufacturer's protocol and the results analyzed using SoftMax Pro V4.8 (full details in Supplementary Materials). IFN-γ levels were below detection limit for most samples and therefore were not analyzed further.
Hepgul N., Cattaneo A., Agarwal K., Baraldi S., Borsini A., Bufalino C., Forton D.M., Mondelli V., Nikkheslat N., Lopizzo N., Riva M.A., Russell A., Hotopf M, & Pariante C.M. (2016). Transcriptomics in Interferon-α-Treated Patients Identifies Inflammation-, Neuroplasticity- and Oxidative Stress-Related Signatures as Predictors and Correlates of Depression. Neuropsychopharmacology, 41(10), 2502-2511.
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