Formalin-fixed tissues were paraffin-embedded and processed for H&E staining by the UTHSCSA Histology/Immunohistochemistry Laboratory. Whole slides were scanned with Aperio microscopy. For Oil Red O staining, the liver tissue samples were embedded in an optical coherence tomography compound and frozen at −80°C before being sectioned and stained with Oil Red O staining. The lipid droplets in the liver were then examined under a microscope.
For IHC, formalin-fixed, paraffin-embedded mouse liver tissues were processed by the UTHSCSA Laboratory Medicine core using standard methods. Tissue sections were fixed in 3% hydrogen peroxide, followed by proteinaceous blocking solution (Avidin/Biotin Blocking Kit). Tissue sections were incubated with the primary antibody, followed by addition of the secondary antibody, with EnVision+ System, HRP polymer and diaminobenzidine (DAB) substrate (Dako), plus DAB sparkle (Biocare). When adequate color development was seen, slides were washed in water to stop the reaction, counterstained with Meyer’s hematoxylin (Dako), and covered with a Permount mounting medium (Richard-Allan Scientific). The micrographs were taken under a light microscope (Zeiss).
For IHC, formalin-fixed, paraffin-embedded mouse liver tissues were processed by the UTHSCSA Laboratory Medicine core using standard methods. Tissue sections were fixed in 3% hydrogen peroxide, followed by proteinaceous blocking solution (Avidin/Biotin Blocking Kit). Tissue sections were incubated with the primary antibody, followed by addition of the secondary antibody, with EnVision+ System, HRP polymer and diaminobenzidine (DAB) substrate (Dako), plus DAB sparkle (Biocare). When adequate color development was seen, slides were washed in water to stop the reaction, counterstained with Meyer’s hematoxylin (Dako), and covered with a Permount mounting medium (Richard-Allan Scientific). The micrographs were taken under a light microscope (Zeiss).