The above total RNA extracted (1.2 µg/sample) was subjected to rRNA depletion by Ribo-Zero rRNA Removal (Human/Mouse/Rat) Kit (Illumina, San Diego, CA). Strand-specific RNA libraries were prepared using TruSeq Stranded Total RNA Sample Preparation Kit (Illumina) without poly-A selection. All assays were performed according to the manufacturer’s instructions. Sequencing was carried out at the Illumina HiSeq2500 platform High Output Mode, in a 2 × 100 base pairs paired-end configuration, with a total of more than 190 M reads per lane (at least 60 M reads per sample).
Hiseq2500 platform high output mode
Manufactured by Illumina
The HiSeq2500 platform in High Output Mode is a high-throughput DNA sequencing system designed for generating large amounts of sequencing data. It utilizes Illumina's proprietary sequencing-by-synthesis technology to produce high-quality sequence reads. The platform is capable of generating up to 600 gigabases of sequencing data per run, making it suitable for a variety of applications that require extensive genomic analysis.
Automatically generated - may contain errors
2 protocols using hiseq2500 platform high output mode
1
Strand-specific RNA Sequencing of Total RNA
2
Profiling DRG Transcriptome Changes in Neuropathic Pain
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The ipsilateral L3/4 DRGs were harvested on day 7 post‐CCI or sham surgery in mice pre‐microinjected with HSV‐DS‐lncRNA or HSV‐Gfp into the ipsilateral L3/4 DRGs. Eight DRGs from four mice were pooled together to achieve enough RNA. Total RNA (1.2 µg/sample) extracted as described above was subjected to rRNA depletion by Ribo‐Zero rRNA Removal (Human/Mouse/Rat) Kit (Illumina, San Diego, CA, USA). Strand‐specific RNA libraries were prepared using TruSeq Stranded Total RNA Sample Preparation Kit (Illumina) without poly‐A selection. All assays were performed according to the manufacturer's instructions. Sequencing was carried out using the Illumina HiSeq2500 platform High Output Mode, in a 2×100 bp paired‐end configuration, with a total of more than 190 m reads per lane (at least 60 m reads per sample).[26
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