Antibodies against total rpS6, phospho-rpS6, Cyclin D1, Cyclin E1, and phospho-4E-BP1 were from Cell Signaling Technology (Beverly, MA). Fluorescein Lotus-tetragonolobus Lectin (LTL) was purchased from Vector Laboratories Inc, (Burlingame, CA). Anti-synaptopodin antibody was from Acris Antibodies GmbH (San Diego, CA). Anti-rabbit HRP and anti-mouse HRP secondary antibodies, anti-rabbit dyLight 488 were from Vector laboratories (Burlingame, CA). Anti-mouse Alexa 594 was from Invitrogen Life Technologies (Carlsbad, CA). Rapamycin was purchased from LC Laboratories (Woburn, MA). Antibodies to β-actin and other chemical reagents were purchased from Sigma-Aldrich (St. Louis, MO).
Fluorescein lotus tetragonolobus lectin
Manufactured by Vector Laboratories
Fluorescein Lotus-tetragonolobus Lectin (LTL) is a plant-derived lectin that binds to specific carbohydrate structures. It is commonly used as a labeling reagent in various research applications, particularly in the visualization and identification of specific cell types or glycoconjugates.
Automatically generated - may contain errors
Lab products found in correlation
Anti mouse alexa594, by Thermo Fisher Scientific (2 mentions)
Cyclin d1, by Cell Signaling Technology (2 mentions)
Antibodies to β actin, by Merck Group (2 mentions)
Anti rabbit hrp and anti mouse hrp secondary antibodies, by Vector Laboratories (2 mentions)
Anti rabbit dylight 488, by Vector Laboratories (2 mentions)
Phospho 4ebp1, by Cell Signaling Technology (2 mentions)
Cyclin e1, by Cell Signaling Technology (2 mentions)
Rapamycin, by LC Laboratories (2 mentions)
3 protocols using fluorescein lotus tetragonolobus lectin
1
Molecular Markers in Kidney Disease
2
Molecular Markers in Kidney Disease
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antibodies against total rpS6, phospho-rpS6, Cyclin D1, Cyclin E1, and phospho-4E-BP1 were from Cell Signaling Technology (Beverly, MA). Fluorescein Lotus-tetragonolobus Lectin (LTL) was purchased from Vector Laboratories Inc, (Burlingame, CA). Anti-synaptopodin antibody was from Acris Antibodies GmbH (San Diego, CA). Anti-rabbit HRP and anti-mouse HRP secondary antibodies, anti-rabbit dyLight 488 were from Vector laboratories (Burlingame, CA). Anti-mouse Alexa 594 was from Invitrogen Life Technologies (Carlsbad, CA). Rapamycin was purchased from LC Laboratories (Woburn, MA). Antibodies to β-actin and other chemical reagents were purchased from Sigma-Aldrich (St. Louis, MO).
3
Bicc1 Protein Expression and Antibody Generation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A pGEX3 GST expression vector (Amersham) was used as the backbone for producing Bicc1 GST-fusion proteins. The cDNA of mouse Bicc1 (Q711-D858) was inserted in-frame into the vector. The mBicc1-fused plasmid was used to transform Rosetta host cells (Novagen) to produce the GST-fusion antigen. The antigen was subcutaneously injected into New Zealand white rabbits, at 0.5 mg per injection. The anti-mBicc1 serum (mBicc1p) was generated as reported in our previous studies [18] (link). All the antisera product and the preimmune serum (Pre-IM) were affinity-purified before performing experiments.
The following antibodies, staining materials, and reagents were used: anti-HA, and anti-Flag, anti-β-actin, and DAPI (Sigma-Aldrich Inc.); anti-Tamm-Horsfall glycoprotein antibody (THG) (Applied Biological Materials Inc.); fluorescein lotus tetragonolobus lectin (LTL) (Vector Laboratories); anti-Aquaporin-2 (AQP2) antibodies (Abcam Inc.); anti-GST antibody and pBABE-Puro retroviral vector (Cell Biolabs Inc.); pCMV-tag4 expression vector (Stratagene Inc.); pSico Lentiviral vector system (Addgene Inc.).
The following antibodies, staining materials, and reagents were used: anti-HA, and anti-Flag, anti-β-actin, and DAPI (Sigma-Aldrich Inc.); anti-Tamm-Horsfall glycoprotein antibody (THG) (Applied Biological Materials Inc.); fluorescein lotus tetragonolobus lectin (LTL) (Vector Laboratories); anti-Aquaporin-2 (AQP2) antibodies (Abcam Inc.); anti-GST antibody and pBABE-Puro retroviral vector (Cell Biolabs Inc.); pCMV-tag4 expression vector (Stratagene Inc.); pSico Lentiviral vector system (Addgene Inc.).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!