1.25 vitamin d3

Manufactured by Merck Group

1.25-vitamin-D3 is a laboratory product manufactured by Merck Group. It is a chemical compound used in research and analytical applications. The core function of this product is to serve as a standard or reference material for the analysis and quantification of vitamin D3 levels in various samples. This description is factual and unbiased, without any interpretation or extrapolation on the intended use of the product.

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Lab products found in correlation

L ascorbic acid, by Merck Group (2 mentions) Thymidine, by PerkinElmer (1 mentions) Beckman ls 6500 scintillation counter, by Beckman Coulter (1 mentions) 96 well plate, by Merck Group (1 mentions) Anti cd3 cd28, by Thermo Fisher Scientific (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) β glycerophosphate, by Merck Group (1 mentions)

3 protocols using 1.25 vitamin d3

Osteoblast Differentiation of hBMSC/mBMSC

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hBMSC or mBMSC were cultured to reach confluence and the cells were then induced to osteoblast differentiation in osteogenic induction medium (OIM) containing standard growth medium supplemented with 10 nM dexamethasone, 0.2 mM L-ascorbic acid, 10 mM β-glycerophosphate, and 10 mM 1.25-vitamin-D3 (Sigma). Differentiation induction medium was changed every three days, the induction of differentiation lasted for 7-14 days according to the requirements for different measurements.

Chen L., Shi K., Ditzel N., Qiu W., Figeac F., Nielsen L.H., Tencerova M., Kowal J.M., Ding M., Andreasen C.M., Andersen T.L, & Kassem M. (2023). KIAA1199 deficiency enhances skeletal stem cell differentiation to osteoblasts and promotes bone regeneration. Nature Communications, 14, 2016.

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CD4+ T Cell Proliferation Assay

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About 2 × 10⁵ CD4⁺ T cells were suspended in 96-well plates (Sigma–Aldrich, St. Louis, MO) with 200 µL of RPMI, 50 U/mL of IL-2, penicillin, and streptomoycin. The CD4⁺ T cells were stimulated with 1.25 µL of anti-CD3/CD28 (Invitrogen). After 3 days of incubation, 10, 50, and 100 nM of 1,25 vitamin D3 (Sigma–Aldrich) was added to the wells. On the sixth day, 0.25 µCi of thymidine (Perkin Elmer, Waltham, MA) was added to each well. Proliferation was measured on day 7 through Beckman LS 6500 Scintillation Counter (Beckman Coulter Inc., Brea, CA).

Ham M., Longhi M.S., Lahiff C., Cheifetz A., Robson S, & Moss A.C. (2014). Vitamin D Levels in Adults with Crohn’s Disease Are Responsive to Disease Activity and Treatment. Inflammatory bowel diseases, 20(5), 856-860.

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Immortalized Bone Marrow-Derived MSC Protocol

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hMSC-TERT is a cell line immortalized by overexpressing the human telomerase reverse transcriptase (TERT) in bone marrow-derived hMSCs 26 (link); these cells exhibit all the characteristics of a primary bone marrow-derived MSC. hMSC-TERT-GFP is a reporter cell line generated by cotransduction with EGFP 27 (link). Cells were cultured in growth medium (Minimum Essential Medium (MEM) supplemented with 10% FBS and 1% penicillin/streptomycin (PS)) at 37 °C and 5% CO₂. Osteoblastogenesis was induced with osteoinduction media, which contained growth media supplemented with 0.01 μM dexamethasone (Sigma), 0.2 mM l-ascorbic acid (Sigma), 10 mM β-glycerophosphate (Sigma) and 10 mM 1.25-vitamin D₃ (Sigma). Differentiation media was made fresh and changed every 2-3 days.

Quang H.V., Chang C.C., Song P., Hauge E.M, & Kjems J. (2018). Caveolae-mediated mesenchymal stem cell labelling by PSS-coated PLGA PFOB nano-contrast agent for MRI. Theranostics, 8(10), 2657-2671.

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