Rabbit anti human cyclin d1

Human glioblastoma cell lines A172, LN229, U87, U251, U343 and U373 were obtained from the cell bank of the Chinese Science Academy, and weres maintained in DMEM supplemented with 10% FBS. Normal human brain tissue was collected from patients who received third ventricular ostomy in our hospital. The use of human tissue in the present study has been reviewed and approved by the Ethics Committee of Renmin Hospital, Hubei University of Medicine, with written informed consent obtained from the patients. Lentiviruses expressing GFP and GFP with miR-16 were constructed by Shanghai Genechem (Shanghai, China). Rabbit anti-human cyclin D1, Bcl-2, WIPI, CD31 and beta-actin polyclonal antibodies were purchased from Santa Cruz (Santa Cruz, USA).

Human breast cancer cell line MCF-7 and SK-BR-3 was a gift from Dr. Song at Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, The University of Rhode Island, USA. Tea Polyphenols was purchased from Sigma (USA). The 5-fluorourocil was purchased from Shanghai Xudong Pharmaceutical. Thiazolyl blue tetrazolium bromide was purchased from Fluka. Rabbit anti- human β-actin polyclonal antibody was purchased from Boster (Wuhan, China). Rabbit anti-human Cyclin D1, Cyclin E, PCNA and Survivin polyclonal antibody was purchased from Santa Cruz (USA).

Rabbit anti-human phospho-IκBα antibody, rabbit anti-human β-actin antibody, and horseradish peroxidase (HRP)-conjugated secondary antibodies were obtained from Cell signaling Technology (Beverly, MA). Rabbit anti-human NF-κB p65 antibody, rabbit anti-human p27Kip1 antibody, rabbit anti-human cyclin D₁, rabbit anti-human cyclin D₂, rabbit anti-human cyclin D₃ antibody, rabbit anti-human cyclin E antibody, and rabbit anti-human ERα antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). The synthetic IKKβ inhibitor IMD-0354 (molecular weight, 384.1) was synthesized and kindly provided by the Institute of Medical Molecular Design Inc. (Tokyo, Japan)31 (link). Unless otherwise indicated, all chemicals used in this study were obtained from Sigma-Aldrich (St. Louis, MO).