Dulbecco’s Modified Eagle Medium (DMEM/HEPES, Cat #12430-054) and Penicillin/Streptomycin (Cat #15240062) were from Gibco (Carlsbad, CA). Fetal bovine serum (FBS) and G418 sulfate were obtained from Invitrogen (Carlsbad, CA). Rabbit antibody against β-actin (Cat #4970S) was from Cell Signaling (Beverly, MA). Rabbit antibody against NHE1 (Cat #ab67314) and rat antibody against CD8 (Cat #ab22378) were from Abcam Ltd. (Cambridge, MA). Rat anti-mouse CD31 (Cat #550274) was from BD Pharmingen (San Jose, CA). Rabbit anti-ionized calcium-binding adapter molecule 1 (iba1) was from Wako (Richmond, VA). PerCP/Cy5.5-CD45, PE-P2RY12, BV421-TGFβ, BV605-TNFα, APC/780-IL-1β, PE/Cy7-IL10, PerCP/Cy5.5-CD8a, APC/Cy7-CD4, Alexa Fluor 700-CD25, PE-FoxP3, BV605-PD-1, PE-Gr-1, APC-NK1.1, and Pacific Blue Granzyme B were obtained from Biolegend (San Diego, CA). eFluor 450-CD16/32, PE/Cy7-CD206, APC-IFNγ was purchased from eBioscience (San Diego, CA). APC-IL-6 were obtained from thermos fisher (Waltham, MA). BUV737-CD11b, Alexa Flour 700-CD86 were obtained from BD Biosciences (San Jose, CA). PE-Ym1were from Abcam Ltd. (Cambridge, MA). Rabbit anti-Laminin (Cat #L9393) and cariporide (HOE642) was purchased from Sigma Chemicals (St. Louis, MO). Anti-mouse PD-1 (RMP1-14) and IgG2a isotype control were from BioXcell (West Lebanon, NH).
Rabbit anti ionized calcium binding adapter molecule 1 iba1
Manufactured by Fujifilm
Sourced in Japan
Rabbit anti-ionized calcium-binding adapter molecule 1 (iba1) is a laboratory reagent used for the detection and study of iba1, a protein involved in the activation and regulation of microglia in the central nervous system. This antibody can be used in various immunodetection techniques such as immunohistochemistry and western blotting to identify and analyze iba1 expression and distribution in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Granzyme b pacific blue, by BioLegend (1 mentions)
Rabbit anti laminin, by Merck Group (1 mentions)
G418 sulfate, by Thermo Fisher Scientific (1 mentions)
Cd4 apc cy7, by BioLegend (1 mentions)
Pe gr1, by BioLegend (1 mentions)
Percp cy5.5 cd45, by BioLegend (1 mentions)
Foxp3 pe, by BioLegend (1 mentions)
Apc ifnγ, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium dmem hepes, by Thermo Fisher Scientific (1 mentions)
Rabbit antibody against β actin, by Cell Signaling Technology (1 mentions)
Bv421 tgfβ, by BioLegend (1 mentions)
Bv605 tnfα, by BioLegend (1 mentions)
Pe cy7 il10, by BioLegend (1 mentions)
Cd25 alexa fluor 700, by BioLegend (1 mentions)
Pd 1 bv605, by BioLegend (1 mentions)
Apc nk1, by BioLegend (1 mentions)
Percp cy5.5 cd8a, by BioLegend (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rat anti mouse cd31, by BD (1 mentions)
2 protocols using rabbit anti ionized calcium binding adapter molecule 1 iba1
1
Molecular Analysis of Cell Signaling Pathways
2
Neuroinflammation Protein Profiling
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Bovine serum albumin (BSA), tribromoethanol, MPTP and probenecid were purchased from Sigma Aldrich (St Louis, MO, USA). Rabbit anti-tyrosine hydroxylase (TH), rat anti-dopamine transporter (DAT), immobilon-P transfer membranes, dimethyl sulfoxide (DMSO) were purchased from Merck Millipore (Burlington, MA, USA). Rabbit anti-ionized calciumbinding adapter molecule-1 (Iba-1) was purchased from Fujifilm Wako (Chuo-Ku, Osaka, Japan). Mouse horseradish peroxidase (HRP)-conjugated β-actin and mouse anti-tumor necrosis factorα (TNF-α) antibodies were purchased from Santa Cruz Biotechnology (Temecula, CA, USA). Goat anti-Glial fibrillary acidic protein (GFAP) was purchased from Invitrogen (Middlesex County, MA, USA). Sodium dodecyl sulfate, protein assay reagent, Tween 20, ammonium persulfate, acrylamide, enzyme-linked chemiluminescence reagent, and skimmed milk were purchased from Bio-Rad Laboratories (Hercules, CA, USA). Anti-rabbit and anti-rat HRP secondary antibodies were purchased from Enzo Life Science, Inc.
(Farmingdale, NY, USA).
(Farmingdale, NY, USA).
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