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Phospho jak1

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Phospho-JAK1 is a laboratory reagent that specifically detects phosphorylated Janus Kinase 1 (JAK1) protein. JAK1 is a member of the Janus Kinase family of tyrosine kinases, which play a crucial role in signal transduction pathways. The Phospho-JAK1 reagent can be used to measure the activation state of JAK1 in various biological samples through techniques such as Western blotting or ELISA.

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Lab products found in correlation

Phospho stat6, by Cell Signaling Technology (1 mentions) Phospho stat5, by Cell Signaling Technology (1 mentions) Phospho jak2, by Cell Signaling Technology (1 mentions) Phospho jak3, by Cell Signaling Technology (1 mentions) Phospho tyk2, by Cell Signaling Technology (1 mentions) Phospho stat2, by Cell Signaling Technology (1 mentions) Flag tag (flag), by Cell Signaling Technology (1 mentions) Phospho stat1, by Cell Signaling Technology (1 mentions) Phospho stat3, by Cell Signaling Technology (1 mentions) Phospho stat5, by R&D Systems (1 mentions) Phospho p70s6kinase, by R&D Systems (1 mentions) Phospho stat3, by R&D Systems (1 mentions) Cd127 fitc, by Miltenyi Biotec (1 mentions) Cd271 apc, by Miltenyi Biotec (1 mentions) Phospho erk1 2, by R&D Systems (1 mentions) β actin, by Merck Group (1 mentions)

2 protocols using phospho jak1

1

Characterizing IL23R Signaling Pathways

Cited 1 time
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Proteins were detected by flow cytometry with Alexa Fluor 488 or phycoerythrin-labelled antibodies to IL23R (FAB41001P, R&D Systems), phospho-JAK1, phospho-JAK2, phospho-JAK3, phospho-TYK2, phospho-STAT1, phospho-STAT2, phospho-STAT3, phospho-STAT5, phospho-STAT6, FLAG (Cell Signaling) or phospho-STAT4 (BD Biosciences).
IL23R was immunoprecipitated using anti-IL23R antibody (Santa Cruz Technology, Santa Cruz, CA) bound to Protein A or Protein G Sepharose (EMD Millipore, Billerica, MA). Associated proteins were blotted with antibodies to JAK2, STAT3 (Cell Signaling), TYK2 (Abcam, Cambridge, MA), IL12Rβ1 (EMD Millipore), JAK3 (Santa Cruz Biotechnology) or IL23A (Proteintech). Control proteins were examined with glyceraldehyde 3-phosphate dehydrogenase or IL23R antibodies (EMD Millipore) as per ref 41 (link).
Sun R., Hedl M, & Abraham C. (2019). IL23 induces IL23R recycling and amplifies innate receptor-induced signalling and cytokines in human macrophages, and the IBD-protective IL23R R381Q variant modulates these outcomes. Gut, 69(2), 264-273.
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2

Western Blot Antibody Compendium

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The following antibodies used for western blot analyses were obtained from Cell Signaling Technology (unless otherwise indicated): phospho-AKTS473 (#9271), phospho-ERK1/2 (#4370), phospho-JAK1 (#3331), phospho-JAK2 (#3771), phospho-MEK1/2 (#9154), phospho-mTOR (#2971), phospho-p70S6Kinase (#9204), phospho-STAT1 (#9167), phospho-STAT3 (#9145), phospho-STAT5 (#9351), phospho-TYK2 (#9321), DYKDDDDK (#2368), CD127 (anti-IL7Ra; R&D Systems, Minneapolis, MN, USA; #MAB306), RAS (Merck Millipore; #05-516) and β-actin (Sigma-Aldrich; #2547). The following antibodies used for flow cytometry were obtained from Miltenyi Biotec: CD127-FITC (#130-094-888) and CD271-APC (#130-091-884).
Canté-Barrett K., Spijkers-Hagelstein J.A., Buijs-Gladdines J.G., Uitdehaag J.C., Smits W.K., van der Zwet J., Buijsman R.C., Zaman G.J., Pieters R, & Meijerink J.P. (2016). MEK and PI3K-AKT inhibitors synergistically block activated IL7 receptor signaling in T-cell acute lymphoblastic leukemia. Leukemia, 30(9), 1832-1843.
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