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2 protocols using reduced gsh luciferin

1

Liver Slice Culture Protocol

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Acetaminophen (cat # A7085) and diclofenac sodium salt (cat # PHR1144) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The V-7 preservation solution was supplied by Vitron (Tucson, AZ, USA) [32 (link)]. Waymouth’s MB 752/1 (without L-glutamine, phenol red and sodium bicarbonate) culture medium and fetal bovine serum were purchased from Invitrogen (Chicago, IL, USA); while L-glutamine, Antibiotic/Antimycotic solution (100×) and gentamicin sulfate were obtained from Sigma-Aldrich (St. Louis, MO, USA). Mixed cellulose-ester IMMOBILIN-NC filters (HATF, 0.45 µm surfactant and triton free, autoclavable), used to support the liver slices on top of the rollers, were obtained from Millipore (Bedford, MA, USA). Dithiobis-nitrobenzoic acid, reduced GSH luciferin, luciferase and ATP were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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2

Liver Slice Preservation and Analysis

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Acetaminophen (cat #A7085), carbamazepine (cat #C8981), cyclosporin A (cat # 30024), dantrolene sodium salt (cat #D9175), diclofenac sodium salt (cat #PHR1144), (+)-etomoxir sodium salt hydrate (cat #E1905), methimazole (cat #M8506) and terbinafine hydrochloride (cat #T8826) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Waymouth’s MB 752/1 (without l-glutamine, phenol red and sodium bicarbonate), and fetal bovine serum were purchased from Invitrogen (Chicago, IL, USA). l-glutamine, Antibiotic/Antimycotic solution and gentamicin sulfate were obtained from Sigma-Aldrich (St. Louis, MO, USA). V‑7 preservation solution was provided by Vitron, Inc. [87 (link)]. Mixed cellulose‑ester IMMOBILIN‑NC filters (HATF, 0.45 µm surfactant and triton free, autoclavable) used to support the liver slices were obtained from Millipore (Bedford, MA, USA). Dithiobis-nitrobenzoic acid, reduced GSH luciferin, luciferase, and ATP were purchased from Sigma-Aldrich.
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