Rabbit polyclonal cox 2 antibody

Curcumin was supplied from LKT Laboratories (St. Paul, MN, USA). TetrahydroCurcumin was prepared by conventional catalytic hydrogenation of Curcumin in the presence of palladium on carbon. DSS (molecular weight, 36,000–50,000) was obtained from ICN Biochemicals, Inc. (Aurora, OH, USA) and MP Biomedical Inc. (Solon, OH, USA). Rabbit polyclonal COX-2 antibody was purchased from Cayman Chemical Co. (Ann Arbor, MI, USA), and iNOS antibody from BD Bioscience (Franklin Lakes, NJ, USA). Primary antibody for β-catenin was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-rabbit and anti-mouse horseradish peroxidase-conjugated secondary antibodies were products of Zymed Laboratories (San Francisco, CA, USA). An oligonucleotide probe containing the NF-κB sequence in the mouse COX-2 promoter region was obtained from Bionics (Seoul, Korea). An oligonucleotide probes harboring the STAT3 consensus sequence was obtained from Santa Cruz Biotechnology.