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2 protocols using mouse anti sc35

1

Antibody Characterization for DNA Damage Response

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Antibodies were as follows: rabbit anti-FANCD2 (NB100-182; Novus) at 1:10,000; rabbit anti-FANCI (A301-254A; Bethyl) at 1:1,000; rabbit anti-SF3B1 (A300-996A; Bethyl) at 1:2,000; rabbit anti-SF3B1 (Eurogentec) at 1:1,000; rabbit anti-SF3A3 (A302-507A; Bethyl) at 1:2,000; rabbit anti-FLAG (F7425; Sigma-Aldrich) at 1:1,000; rabbit anti-DHX15 (A300-389A; Bethyl) at 1:5,000, mouse anti-SC35 (556363; BD Pharmingen) at 1:500; rabbit anti-U2AF65 (ab37530; Abcam) at 1:250; rabbit anti-pSer345(Chk1) (2348L, rabbit; Cell Signaling Technology) at 1:1,000; mouse anti-Chk1 (Sc-8408; Santa Cruz Biotechnology) at 1:1,000; mouse anti-tubulin (T5168; Sigma-Aldrich) at 1:10,000; anti-rabbit HRP-linked (7074; Cell Signaling Technology) at 1:2,500; anti-mouse HRP-linked (7076; Cell Signaling Technology) at 1:2,500; rabbit anti-Nbs1 (NB100-143; Novus Biologicals) at 1:1,000; mouse anti-PCNA (P8825; Sigma-Aldrich) at 1:3,000; rabbit anti-TopBP1 (A300-111A; Bethyl) at 1:1,000; rabbit anti-RNA-PolII (N-20) (sc-899; Santa Cruz Biotechnology) at 1:1,000; rabbit anti-TFIP11 (A302-548A; Bethyl) at 1:1,000; rabbit anti-SPF45 (A302-548A; Bethyl) at 1:1,000; rabbit anti-FANCA (A301-980A; Bethyl) at 1:2,000; and rabbit anti-PRP8 (GTX108046; GeneTex) at 1:1,000.
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2

Immunofluorescent Labeling of Nuclear Proteins

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Antibodies were as follows: mouse anti-SC35 (556363; BD Pharmingen) at 1:300; rabbit anti-SF3B1 (Eurogentec) at 1:200; and Alexa Fluor 546 goat anti–mouse IgG and Alexa Fluor 488 goat anti–rabbit IgG (A11030 and A11008; Invitrogen) at 1:500.
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