6-well (3516), 96-well tissue culture (3596), 96-well round bottom (3799), T75 (430641) flasks, and 35 um filter cap FACS tubes (352235) were from Corning. DMEM (11960), Penicillin-Streptomycin (15070, 5000 U/mL), Glutamax (100X, 35050-061), Sodium Pyruvate (11360, 100 mM), and 0.25% trypsin/1 mM EDTA (25200) were from Invitrogen. Fetal Bovine Serum (SH30396.03) was from Hyclone. AO/PI (F23001) and Luna (L12002) slides were from Logos Biosystems. CD44-APC (clone G44-26; #559942) was from BD Biosciences. DMSO (D2650), resazurin (R7017), and DAPI (D8417) were from Sigma. EREG ELISA (SEB945HU) was from Cedarlane. RNeasy Mini Kit (74106) was from Qiagen. RNA 6000 Pico and Nano Kits were from Agilent. Ovation Pico WTA System Kit was from NuGEN. Human Gene ST 2.1 microarrays were from Affymetrix. Clinical-grade cisplatin (Teva Pharmaceuticals; 1mg/mL) was obtained from University of Michigan Hospital Pharmacy. BGJ398 (S2183) was from Selleckchem and dissolved in DMSO to 1.78 mM. DEAB (01705) and Aldefluor Assay Buffer (01702) were from StemCell Technologies. Aldefluor reagent, BAAA-DA, was synthesized by the University of Michigan Vahlteich Medicinal Chemistry Core.
Human gene st 2.1 microarrays
Manufactured by Thermo Fisher Scientific
The Human Gene ST 2.1 microarrays are a high-density oligonucleotide microarray designed to measure the expression of protein-coding and non-coding transcripts across the human genome. The array contains probes targeting over 40,000 well-annotated genes and 10,000 additional coding and non-coding transcripts. The core function of this product is to provide a comprehensive and unbiased platform for gene expression analysis in human samples.
Automatically generated - may contain errors
2 protocols using human gene st 2.1 microarrays
1
Characterization of Cancer Cell Lines
2
RNA Expression and Autoantibody Profiling
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RNA was extracted from whole blood or from purified leukocyte subsets as described previously 32 , and hybridised to Human Gene ST 2.1 microarrays (Affymetrix). Microarray data are available in the ArrayExpress database (http://www.ebi.ac.uk/arrayexpress) under accession number E-MTAB-5906.
Patient serum was hybridised to ProtoArray (Invitrogen) protein microarrays according to manufacturer's instructions. Details of gene expression and protein array analyses are in the supplementary appendix.
Patient serum was hybridised to ProtoArray (Invitrogen) protein microarrays according to manufacturer's instructions. Details of gene expression and protein array analyses are in the supplementary appendix.
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