Ceftriaxone

Manufactured by Liofilchem

Sourced in Italy

Ceftriaxone is a broad-spectrum cephalosporin antibiotic used in the laboratory setting. It is a sterile powder that can be reconstituted for antimicrobial susceptibility testing or other microbiological applications.

Automatically generated - may contain errors

Lab products found in correlation

4 protocols using ceftriaxone

Antibiotic Susceptibility Testing with E-Test

Check if the same lab product or an alternative is used in the 5 most similar protocols

Because the disk diffusion method allows to analysis of the impact of a single concentration of an antibiotic/disk, in this experiment E-test strips with exponentially decreasing antibiotic gradient were applied. In this investigation line, the antibiotics were selected whose application, coupled with exposure to RMF, caused the largest and the smallest differences in the zones of growth inhibition in the disc diffusion method (compared to unexposed controls). The E-tests containing cefoxitin, amoxicillin, imipenem, ceftriaxone, cefuroxime, ceftazidime and cefepime were obtained from Liofilchem (Roseto degli Abruzzi, Italy), whereas the E-test containing meropenem was purchased from BioMérieux (Craponne, France).
Similarly, as in the case of the disc diffusion method, the optimal RMF frequency and exposure time, as well as unexposed controls were used. The analyses were performed on M-H agar in accordance with the E-test manufacturer’s recommendations.

Woroszyło M., Ciecholewska-Juśko D., Junka A., Drozd R., Wardach M., Migdał P., Szymczyk-Ziółkowska P., Styburski D, & Fijałkowski K. (2021). Rotating Magnetic Field Increases β-Lactam Antibiotic Susceptibility of Methicillin-Resistant Staphylococcus aureus Strains. International Journal of Molecular Sciences, 22(22), 12397.

+ Open protocol

+ Expand

Detecting Neisseria gonorrhoeae via PCR

Check if the same lab product or an alternative is used in the 5 most similar protocols

A direct PCR was performed in the KEMRI-NUITM laboratory to confirm the presence of Neisseria gonorrhoeae. The direct PCR was done using the mighty amp reagents and the simpliprep machine. Specifically designed primers, forward-CGGCAGCATTCAATTTGTTAAAGC and reverse-CGCCATTTTTGTA, which were to yield a PCR product of 162 base pairs each, were used [14 ]. The thermal cycle conditions for the simpliprep machine were: 94°C, 60 sec (denaturation), 59°C, 60 secs (annealing), 72°C, 26 sec/max 429bp (1 min. kbp), (elongation), 72°C, 10 minutes (extended elongation). When the reaction was over, the samples were loaded on gel electrophoresis, stained, and viewed under ultraviolet light (UV).
The antimicrobial susceptibility testing was done by disk diffusion using 13 antimicrobials from Liofilchem Company (Ciprofloxacin (CIP,30μg), Ceftriaxone (CRO,30μg), Cefepime (FEP,25μg), Cefotaxime (CTX,30μg), Cefuroxime (CXM,30μg), Cefazolin (KZ,30μg), Ceftazidime (CAZ,30μg), Cefoxitin (FOX,30μg), Tetracycline (TE,30μg), Ampicillin (AMP,10μg), Amikacin (AK,30μg), Gentamycin (CN,30μg) and Amoxillin (AUG,30μg).The antimicrobial discs were inoculated onto a culture of Neisseria gonorrhoeae and incubated for 24–48 hours. The zone of inhibition was measured and interpreted using the CLSI 2016 guidelines.

Abdullahi A., Nzou S.M., Kikuvi G, & Mwau M. (2022). Neisseria gonorrhoeae infection in female sex workers in an STI clinic in Nairobi, Kenya. PLoS ONE, 17(2), e0263531.

+ Open protocol

+ Expand

Pneumococcal Susceptibility Testing

Check if the same lab product or an alternative is used in the 5 most similar protocols

All isolates were identified according to the defined protocol of the Swiss National Center for Pneumococci [12 (link)]. Isolates were tested for penicillin and ceftriaxone susceptibility by using two different methods: the BMD method with cation-adjusted Mueller-Hinton broth supplemented with 5% lysed horse blood and the Etest method (penicillin from bioMérieux SA, Marcy-l’Étoile, France; ceftriaxone from Liofilchem, Roseto degli Abruzzi, TE, Italy) on MHA with 5% mechanically defibrinated horse blood and 20 μg/mL nicotinamide adenine dinucleotide [11 ]. The following CLSI categorization from 2022 was used for S. pneumoniae and meningitis [11 ]: penicillin ≤ 0.06 mg/L = susceptible, ≥0.12 mg/L = resistant; ceftriaxone ≤ 0.5 mg/L = susceptible, ≥2 mg/L = resistant.

Raemy S., Casanova C., Baldan R., Barreto E., Tande A.J., Endimiani A., Leib S.L., Fischer U, & Sendi P. (2023). Penicillin-Susceptible Streptococcus pneumoniae Meningitis in Adults: Does the Ceftriaxone Dosing Matter?. Antibiotics, 12(5), 878.

+ Open protocol

+ Expand

Antimicrobial Susceptibility Testing of Salmonella Choleraesuis

Check if the same lab product or an alternative is used in the 5 most similar protocols

S. Choleraesuis isolates were tested for susceptibility to antimicrobial agents using the agar disk diffusion method (Kirby Bauer test) following the guidelines and criteria of the Clinical and Laboratory Standards Institute (CLSI) [26 ]. The antibiotics tested (Oxoid, England) were ampicillin (10 µg), amoxicillin–calvulanic acid (20/10 µg), aztreonam (30 µg), cefepime (30 µg), ceftazidime (30 µg), cefoxitin (30 µg), cefotaxime (30 µg), cefpodoxime (10 µg), cephalothin (30 µg), cefuroxime (30 µg), ceftriaxone (30 µg), ceftiofur (30 µg), ciprofloxacin (5 µg), chloramphenicol (30 µg), nalidixic acid (30 µg), norfloxacin (10 µg), streptomycin (10 µg), tetracycline (30 µg), and trimethoprim/sulfamethoxazole (25 µg). Furthermore, cefotaxime-resistant S. Choleraesuis isolates were screened for ESBL producers by the combination disk method (CLSI, 2008) [12 (link)]. The MICs for ciprofloxacin (32–0.002 µg/mL), cefotaxime (256-0.016 µg/mL), ceftazidime (256-0.016 µg/mL), ceftriaxone (256-0.016 µg/mL) and cefpodoxime (256-0.016 µg/mL) were determined using the Liofilchem MIC test strip (Liofilchem, Roseto degli Abruzzi, Italy), according to the manufacturer’s instructions. Escherichia coli ATCC 25922 was used as the quality control strain for antimicrobial susceptibility testing.

Sriyapai P., Pulsrikarn C., Chansiri K., Nyamniyom A, & Sriyapai T. (2021). Molecular Characterization of Cephalosporin and Fluoroquinolone Resistant Salmonella Choleraesuis Isolated from Patients with Systemic Salmonellosis in Thailand. Antibiotics, 10(7), 844.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!