U0126

Manufactured by Avantor

U0126 is a cell-permeable inhibitor of the dual-specificity mitogen-activated protein kinase kinases MEK1 and MEK2. It blocks the activation of the extracellular signal-regulated kinases ERK1 and ERK2, thereby inhibiting the ERK signaling pathway.

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Lab products found in correlation

Ly294002, by Avantor (2 mentions) Verapamil, by Merck Group (1 mentions) Dynasore, by Bio-Techne (1 mentions) Sb239063, by Merck Group (1 mentions) Bi6015, by Cayman Chemical (1 mentions) Sp600125, by Avantor (1 mentions) Sanguinarine, by Bio-Techne (1 mentions) Cox 4, by Cell Signaling Technology (1 mentions) P erk, by Merck Group (1 mentions) P jnk, by Merck Group (1 mentions) Lapatinib, by Selleck Chemicals (1 mentions) Lithium chloride (licl), by Merck Group (1 mentions) Sb415286, by Merck Group (1 mentions) E cadherin hecd1, by Abcam (1 mentions) β actin ac 15, by Merck Group (1 mentions)

3 protocols using u0126

Piezo1 Expression Regulation in Chondrocytes

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Chondrocytes were treated with compounds for 1∼2 h during Fura-2-AM loading process: GsMTx4 peptide (2 μM, provided by Philip Gottlieb, State University of New York at Buffalo), dynasore (5 μM, Tocris), and verapamil (0.5 μM, Sigma). To identify signal transduction mechanisms and transcription factors that regulate IL-1α–induced Piezo1 expression, inhibitors were coincubated with IL-1α (1 ng/mL) for 3 d. Inhibitors used were SGCCBP30 (10 to 50 μM, Cayman Chemical), BI6015 (10 to 50 μM, Cayman), YC-1 (10 μM, Cayman), nuclear factor of activated T cells (NFAT) inhibitor (10 μM, Cayman), SB203580 (10 μM, VWR), SB239063 (10 μM, Sigma Aldrich), LY294002 (10 μM, VWR), PI828 (10 μM, Fisher Scientific), SP600125 (10 μM, VWR), and U0126 (10 μM, VWR).

Lee W., Nims R.J., Savadipour A., Zhang Q., Leddy H.A., Liu F., McNulty A.L., Chen Y., Guilak F, & Liedtke W.B. (2021). Inflammatory signaling sensitizes Piezo1 mechanotransduction in articular chondrocytes as a pathogenic feed-forward mechanism in osteoarthritis. Proceedings of the National Academy of Sciences of the United States of America, 118(13), e2001611118.

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Signaling Pathway Inhibitors and Antibodies

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U0126 was purchased from VWR International (West Chester, PA), JC-1 from Invitrogen (Grand Island, NY), Sanguinarine from Tocris Biosciences (Minneapolis, MN), and Lapatinib from Selleckchem (Houston, TX). Antibodies for MKP1, ERK, pERK, JNK, pJNK, HER2, and TOM40 were purchased from Sigma (St. Louis, MO); COXIV and pMKP1 were purchased from Cell Signaling (Beverly, MI).

Candas D., Lu C.L., Fan M., Chuang F.Y., Sweeney C., Borowsky A.D, & Li J.J. (2014). Mitochondrial MKP1 is a target for therapy-resistant HER2-positive breast cancer cells. Cancer research, 74(24), 7498-7509.

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Molecular Signaling Pathway Inhibitor Protocol

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Pharmacological inhibitors PD153035, U0126 and LY294002 were purchased from VWR (Merck). GSK3β inhibitors SB415286 and LiCl were from Sigma Aldrich. The antibodies used were against; active β-catenin dephosphorylated on Ser37 and Thr41 (8E7; a kind gift from Hans Clevers, Utrecht University), total β-catenin (C2206; Sigma Aldrich), β-actin (AC-15; Sigma), E-cadherin (HECD-1; Abcam), total ERK (16; Transduction Laboratories), phospho-42/44 MAPK (D13.14.4E; Cell Signalling Technology), AKT (7; BD Biosciences), phospho-473 AKT (clone D9E; Cell Signalling Technology) and phospho-9 GSK3β (AB30619; Abcam). The secondary antibodies were from Invitrogen. The secondary antibodies for immunofluorescence microscopy were Alexa-Fluor-488-conjugated goat anti-mouse-IgG and Alexa-Fluor-594-conjugated goat anti-rabbit-IgG, and those used for western blotting were Alexa-Fluor-680-conjugated goat anti-mouse-IgG, Alexa-Fluor-800-conjugated goat anti-rabbit-IgG and Alexa-Fluor-680-conjugated donkey anti-goat-IgG.

Georgopoulos N.T., Kirkwood L.A, & Southgate J. (2014). A novel bidirectional positive-feedback loop between Wnt–β-catenin and EGFR–ERK plays a role in context-specific modulation of epithelial tissue regeneration. Journal of Cell Science, 127(13), 2967-2982.

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