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Sml0341

Manufactured by Merck Group
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SML0341 is a laboratory equipment product from Merck Group. It is a device designed for specific laboratory functions. No further details about its core function or intended use can be provided in an unbiased and factual manner.

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Lab products found in correlation

Mcf 7 breast, by American Type Culture Collection (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) I0634, by Merck Group (1 mentions) B0560, by Merck Group (1 mentions) L7260, by Merck Group (1 mentions) Ct04 a, by Cytoskeleton (1 mentions) Y0503, by Merck Group (1 mentions) C9754, by Merck Group (1 mentions)

2 protocols using sml0341

1

Establishing and Treating Cell Lines

Cited 1 time
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Human HT-1080 fibrosarcoma, A431 epidermoid carcinoma, MCF-7 breast cancer, and human embryonic kidney (HEK) 293T cells were obtained from the American Type Culture Collection and cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing glucose (4.5 g/liter), l-glutamine, and sodium pyruvate (11995073, Gibco). Medium was supplemented with 10% heat-inactivated FBS (16140071, Gibco) and 1% penicillin-streptomycin (10,000 U/ml; 15140122, Gibco). Cells were maintained at 37°C with 5% CO2. A431 cells expressing GFP-E-cad were a gift from V. Bruntons (EH4 2XR, University of Edinburgh, UK) (63 (link)). In select experiments, cells were treated with the following pharmacological agents: Y-27632 (20 μM; Y0503, Sigma-Aldrich), ionomycin (0.5 and 1 μM; I0634, Sigma-Aldrich), blebbistatin (50 μM; B0560, Sigma-Aldrich), Rho inhibitor I (1 μg/ml; CT04-A, Cytoskeleton Inc.), LPA (50 μM; L7260, Sigma-Aldrich), paclitaxel (taxol equivalent) (1 μM; P3456, Thermo Fisher Scientific), colchicine (125 μM; C9754, Sigma-Aldrich), and importazole (50 μM; SML0341, Sigma-Aldrich).
Law R.A., Kiepas A., Desta H.E., Perez Ipiña E., Parlani M., Lee S.J., Yankaskas C.L., Zhao R., Mistriotis P., Wang N., Gu Z., Kalab P., Friedl P., Camley B.A, & Konstantopoulos K. (2023). Cytokinesis machinery promotes cell dissociation from collectively migrating strands in confinement. Science Advances, 9(2), eabq6480.
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2

Dissolution of Nuclear Import Inhibitors

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Inhibitor of Nuclear Import-43 (INI-43) (Sigma-Aldrich #SML1911) and Importazole (IPZ) (Sigma-Aldrich #SML0341) were dissolved in DMSO to stock concentrations of 25 mM and 50 mM, respectively for in vitro experiments. For in vivo studies, INI-43 was dissolved in 10% DMSO/20% PEG400/70% PBS.
Kelenis D.P., Rodarte K.E., Kollipara R.K., Pozo K., Choudhuri S.P., Spainhower K.B., Wait S.J., Stastny V., Oliver T.G, & Johnson J.E. (2022). Inhibition of karyopherin β1-mediated nuclear import disrupts oncogenic lineage-defining transcription factor activity in small cell lung cancer. Cancer research, 82(17), 3058-3073.
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