Cell b basic imaging software

Manufactured by Olympus

Sourced in Japan

Cell B Basic Imaging Software is a software solution for basic image acquisition and analysis. It provides fundamental tools for capturing, managing, and processing digital images.

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Lab products found in correlation

Sp 350 digital camera, by Olympus (1 mentions) Bx51 microscope, by Olympus (1 mentions) Cc1 solution, by Roche (1 mentions) Sc50 camera, by Olympus (1 mentions) Benchmark ultra, by Roche (1 mentions) Ack buffer, by Quality Biological (1 mentions) Monoclonal anti phospho akt ser473 antibody, by Cell Signaling Technology (1 mentions)

4 protocols using cell b basic imaging software

Histological Analysis of Parasitic Infection

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For histology, tissue samples collected from the cases 2, 3 and 4 were fixed in 10 % phosphate-buffered formalin (pH = 7.0) for 24 h, routinely processed, embedded in paraffin wax, cut into 2–3 μm sections, and stained with hematoxylin and eosin (H&E). Samples were examined using an Olympus BX51 microscope. Photomicrographs and measurements of the parasites for morphologic identification were taken using an Olympus SP 350 digital camera and Cell^B basic imaging software (Olympus Corporation, Japan).

Taulescu M.A., Negoescu A., Ungur A., Toma C., Ionică A.M., Gal C., Sandu I., Buzdea A., Tutuneanu A., Turcitu M., Horvat I.E, & Deak G. (2023). Is the Angiostrongylus vasorum infection in domestic dogs underestimated or misdiagnosed? A comprehensive presentation of four lethal cases. Frontiers in Veterinary Science, 10, 1146713.

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Quantifying Lymphatic Vessel Density in IgA Nephropathy

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All biopsies were processed for light and immunofluorescence microscopy using standard techniques, examined by an experienced nephropathologist, and interpreted according to the Oxford Classification of IgAN.
The morphometric analysis of lymphatic vessels was performed on formalin-fixed, paraffin-embedded tissue sections using BenchMark ULTRA (Ventana) and commercially available antibody (podoplanin: D2-40, mouse monoclonal antibody, ready to use, Cell Marque). The D2-40 antibody was used after standard antigen retrieval (CC1 solution Ventana; 36 minutes, 95 °C).
Podoplanin is a ∼38 kD O-linked transmembrane sialoglycoprotein expressed on the endothelium of lymphatic capillaries, but not in the blood vasculature. Clone D2-40 recognizes podoplanin in normal and neoplastic tissues, and it has been used for the identification of lymphatic invasion in a variety of cancers.¹⁰ ^,¹¹
Lymphatic vessels were counted only in the cortex, and their areas were measured, using an Olympus Cell B Basic Imaging Software and the Olympus SC50 camera attached to an BX51 microscope. Lymphatic areas were defined as vascular areas delineated by D2-40–positive staining. Evaluated parameters included microvessel areas, the total area of the biopsy analyzed, the density of lymphatic vessels (number per mm² biopsy area), and the proportion of the examined area occupied by lymphatic vessels.

Rodas L., Barnadas E., Pereira A., Castrejon N., Saurina A., Calls J., Calzada Y., Madrid Á., Blasco M., Poch E., García-Herrera A, & Quintana L.F. (2022). The Density of Renal Lymphatics Correlates With Clinical Outcomes in IgA Nephropathy. Kidney International Reports, 7(4), 823-830.

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Evaluating Anti-CD20 Therapy in Xenograft

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SCID mice were preconditioned with 25 mg/kg of busulfan 24hr before inoculation via tail vein of MEC2 cells (10⁷ cells per mouse), following a protocol approved by the Animal Testing Ethic committee of the University of Barcelona. One week later, mice were randomly assigned into cohorts of 6-7 mice. A saturating loading dose of 20 mg/kg DARA or isotype control i.p. was given on day 7 and thereafter 10 mg/kg weekly for 3 weeks. Mice were sacrificed if they lost 15-20% of weight and/or showed signs of disease. Survival studies were extended up to day 90 when the study was terminated. The presence of tumor cells was evaluated first macroscopically and then by flow cytometry. Cells from infiltrated organs were obtained by tissue homogenization. BM cells were obtained after flushing the femoral and tibia bones with RPMI 1640 media. These samples were filtered through 70 μm nylon sieves (BD Falcon). Erythrocytes were lysed using ACK buffer (Quality Biological Inc.).The cells were labeled with huCD45/CD19/CD5 antibodies and analyzed by flow cytometry. Organ samples were snap-frozen in OCT medium (Sakura Tissue Tek) or formalin fixed and embedded in paraffin. Tissue sections were stained with H&E and CD19 (Dako) antibody and evaluated by Cell B Basic Imaging Software (Olympus).

Matas-Céspedes A., Vidal-Crespo A., Rodriguez V., Villamor N., Delgado J., Giné E., Roca-Ho H., Menéndez P., Campo E., López-Guillermo A., Colomer D., Roué G., Wiestner A., Parren P.W., Doshi P., van Bueren J.L, & Pérez-Galán P. (2016). The human CD38 monoclonal antibody daratumumab shows anti-tumor activity and hampers leukemia-microenvironment interactions in chronic lymphocytic leukemia. Clinical cancer research : an official journal of the American Association for Cancer Research, 23(6), 1493-1505.

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Evaluating Tumor Angiogenesis and Signaling

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Fifty-two DLBCL samples were included in tissue microarrays using duplicated cores of 1 mm per tumor sample. CD31⁺ microvascular density was stained and quantified as previously described.^{31 (link)} Microvascular density values were grouped in quartiles and considered high or low when above or below the 50^th percentile. Xenograft tumor samples were stained for phosphohistone H3, cleaved caspase-3 and MYC, as previously described.^{32 (link)} Phospho-Akt was detected using a monoclonal anti-phospho-Akt-Ser473 antibody (Cell Signaling Technology). Preparations were evaluated on a DP70 or a BX51 microscope using Cell B Basic Imaging Software (Olympus).

Recasens-Zorzo C., Cardesa-Salzmann T., Petazzi P., Ros-Blanco L., Esteve-Arenys A., Clot G., Guerrero-Hernández M., Rodríguez V., Soldini D., Valera A., Moros A., Climent F., González-Barca E., Mercadal S., Arenillas L., Calvo X., Mate J.L., Gutiérrez-García G., Casanova I., Mangues R., Sanjuan-Pla A., Bueno C., Menéndez P., Martínez A., Colomer D., Tejedor R.E., Teixidó J., Campo E., López-Guillermo A., Borrell J.I., Colomo L., Pérez-Galán P, & Roué G. (2019). Pharmacological modulation of CXCR4 cooperates with BET bromodomain inhibition in diffuse large B-cell lymphoma. Haematologica, 104(4), 778-788.

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