Cartilage specimens sections were put into 1% alcian blue (Sigma, St. Louis, MO, USA) for 50 min. In which, alcian blue was diluted in 3% acetic acid (Beyotime, Shanghai, China). Then sections were washed in water. alcian blue score was evaluated following with previous study base on the grading system from zero to three: 0, negative; 1, weakly positive; 2, positive; 3, strongly positive (Gutiérrez et al. 2012 (link)). All evaluations were performed in a double-blind manner by two expert (Liao and Zhang).
Alcian blue
Manufactured by Beyotime
Sourced in China
Alcian blue is a staining dye used in histology and microscopy to detect the presence of acidic polysaccharides, such as glycosaminoglycans. It selectively binds to these molecules, allowing their visualization and identification in tissue samples.
Automatically generated - may contain errors
Lab products found in correlation
Alcian blue, by Merck Group (1 mentions)
Alizarin red s, by Beyotime (1 mentions)
Tgf β3, by Merck Group (1 mentions)
Oil red o, by Wuhan Servicebio Technology (1 mentions)
Rapi lysate, by Beyotime (1 mentions)
Bca protein concentration determination kit, by Beyotime (1 mentions)
1 1 dioctadecyl 3 3 3 3 tetramethylindotricarbocyaine iodide dir, by Yeasen (1 mentions)
4 protocols using alcian blue
1
Alcian Blue Cartilage Staining Protocol
2
Skeletal Staining and Clearing
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Mice were eviscerated, the skin was removed, and the resulting samples were transferred into acetone for 48 h after overnight fixation in 95% ethanol. Skeletons were then stained in Alcian blue and Alizarin Red S solution (Beyotime, Cat# C0148S, China) for 3 days at 37 °C and sequentially cleared in 1% KOH. Skeletons were then replaced in 1% KOH/20% glycerol for 3 days and passed through increasing concentrations of 1:1 glycerol/ethanol solution (20%, 50%, and 100%) for 1 day. Finally, the stained skeletons were dehydrated in glycerol for imaging and storage.
3
Chondrogenic Differentiation of CP-M176 Cells
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CP‐M176 cells were inoculated at a density of 1 × 105 per well into 48‐well plates. When the cells grew to 80% confluence, the original medium was discarded, and the chondrogenic medium was added to induce chondrogenic differentiation. The chondrogenic induction medium consisted of the macrophage‐conditioned medium, 1 mm sodium pyruvate, vitamin C (50 µg mL−1), 10−7 m dexamethasone, 1% Insulin‐Transferrin‐SelenIum (Beyotime; China), and TGF‐β3 (10 ng mL−1) (Merck; Germany). After 14 days of culture, cells were stained using Alcian blue (Beyotime; China) and Oil Red O (Servicebio; China). Micrographs were analyzed for optical density using ImageJ.
4
Nanoparticle-Based Drug Delivery Protocol
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PPTT-NPs and PP-NPs (PNS-PLGA nanoparticles) were prepared by our laboratory.24 (link) DAPI, PMSF, 4% tissue cell fixative, 1×PBST buffer, 1×PBS buffer, trypsin, anti-fluorescence attenuation mounting tablets, ketoconazole and neutral gum were purchased from Soleibao Biotechnology Co., Ltd. BCA protein concentration determination kit, RAPI lysate and Alcian Blue were purchased from Beyotime Co., Ltd. 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindotricarbocyaine iodide (DiR) was purchased from Yeasen Biotechnology Co., Ltd. HE staining reagent was purchased from Nanchang Yu Lu Experimental Equipment Co., Ltd. The mixed SD rat liver microsomes, CYP450 enzyme metabolism phenotype research kit was purchased from Beijing Huizhi Taikang Pharmaceutical Technology Co., Ltd.
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