For additional characterization of satellite cells, we stained for lineage negative; CXCR4 and Itgb1 double positive cells. PE-Cy7 anti-mouse TER-119 (clone TER-119), APC anti-mouse/rat CD29 (clone eBioHMb1-1), and PE-Cy7 anti-mouse Ly6A/E (Sca-1) (clone D7) from eBioscience (San Diego, CA); PE-Cy7 rat anti-mouse CD11b (clone M1/70), PE-Cy7 CD45, and Biotin rat anti-mouse CD184 from BD Biosciences antibodies were incubated with samples on ice for 1 h. Additionally, we stained for lineage negative; CD34, Itga7 double-positive cells. PE-Cy7 Rat anti-mouse CD11b (clone M1/70), PE-Cy7 anti-mouse Ly6A/E (Sca-1) (clone D7), and Biotin anti-mouse CD34 (clone RAM34) from eBioscience (San Diego, CA) and Itga7 PE (clone R2F2) from AbLab (Vancouver, B.C., Canada) antibodies were incubated for 1 h on ice. After this incubation, samples were washed and resuspended with FACS staining medium. PE or APC conjugated Streptavidin reagents, respectively, were incubated on ice for 20 min. Samples were washed and resuspended with FACS staining medium for FACS analysis.
Arpke R.W., Shams A.S., Collins B.C., Larson A.A., Lu N., Lowe D.A, & Kyba M. (2021). Preservation of satellite cell number and regenerative potential with age reveals locomotory muscle bias. Skeletal Muscle, 11, 22.
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