4T1 cancer cells (1 × 105) seeded in confocal petri dish were incubated with PM, PM@RM and PM@RM-T7 (at the equivalent mPDA = 200 μg/mL, Met = 60 μg/mL) for 6 h. Afterward, the cells were laser irradiated (808 nm, 1.0 W/cm2, 5 min) or not. The medium was changed to a fresh medium. After another incubation for 24 h, 4T1 cells were washed with PBS (pH 7.4) and incubated with 5% bovine serum albumin for 30 min to block unspecific binding of antibodies. Then, the cells were respectively incubated with anti-calreticulin (CRT) and anti-heat shock protein (HSP70) antibodies (Cell Signaling Technology, Danvers, MA, USA) overnight at 4 °C, followed by incubation with the corresponding secondary antibodies for 2 h at 37 °C in the dark. Finally, the cells were visualized under a CLSM (Zeiss) system. The procedures of immunofluorescence (IF) staining of PD-L1 (Beyotime) were the same as the above.
Anti calreticulin crt
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-calreticulin (CRT) is a laboratory product used for the detection and analysis of calreticulin, a ubiquitous calcium-binding chaperone protein found in the lumen of the endoplasmic reticulum. It functions as a molecular chaperone, facilitating the folding and assembly of newly synthesized proteins.
Automatically generated - may contain errors
Lab products found in correlation
Anti heat shock protein hsp70 antibodies, by Cell Signaling Technology (2 mentions)
Pd l1, by Beyotime (2 mentions)
L arg, by Solarbio (1 mentions)
Edu apollo567 kit, by RiboBio (1 mentions)
Anti cd8 pe antibody, by Thermo Fisher Scientific (1 mentions)
Triton x 100, by Solarbio (1 mentions)
Lecithin, by Maclin Biochemical Technology (1 mentions)
Anti cd3 fitc, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
Dcfh da, by Beyotime (1 mentions)
Matrigel, by Corning (1 mentions)
Tween 20, by Solarbio (1 mentions)
Daf fm da, by Beyotime (1 mentions)
3 protocols using anti calreticulin crt
1
Immunogenic Cell Death Analysis
2
Immunogenic Cell Death Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
4T1 cancer cells (1 × 105) seeded in confocal petri dish were incubated with PM, PM@RM and PM@RM-T7 (at the equivalent mPDA = 200 μg/mL, Met = 60 μg/mL) for 6 h. Afterward, the cells were laser irradiated (808 nm, 1.0 W/cm2, 5 min) or not. The medium was changed to a fresh medium. After another incubation for 24 h, 4T1 cells were washed with PBS (pH 7.4) and incubated with 5% bovine serum albumin for 30 min to block unspecific binding of antibodies. Then, the cells were respectively incubated with anti-calreticulin (CRT) and anti-heat shock protein (HSP70) antibodies (Cell Signaling Technology, Danvers, MA, USA) overnight at 4 °C, followed by incubation with the corresponding secondary antibodies for 2 h at 37 °C in the dark. Finally, the cells were visualized under a CLSM (Zeiss) system. The procedures of immunofluorescence (IF) staining of PD-L1 (Beyotime) were the same as the above.
3
Multimodal Reagent Procurement Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tween 20, 0.1% Triton X-100, PTX and L -Arg were obtained from Solarbio (Beijing, China). O-CMC was supplied by Santa Cruz (California, USA). PFH was obtained from Aladdin (Shanghai, China). Lecithin were purchased by Macklin (Shanghai, China). DCFH-DA, DAF-FM DA, DAPI, DiI and DiO were supplied by Beyotime (Shanghai, China). Matrigel was achieved from Corning (New York, USA). Anti-calreticulin (CRT) and anti-high mobility group box 1 (HMGB-1) antibody were supplied by Cell Signaling Technology (Boston, USA). EdU Apollo567 kit was supplied by RiboBio (Guangzhou, China). Anti-CD3-FITC and anti-CD8-PE antibody were supplied by eBioscience (MA, USA). Anti-CD4-APC were supplied by Elabscience (Wuhan, China). ELISA kits were supplied by BOSTER (Wuhan, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!